A COMPARATIVE STUDY OF EFFECT OF TYPES OF HIBISCUS SABDARIFFA EXTRACTS AND SELECTIVE ANTIBIOTICS ON CLINICAL AND STANDARD STRAINS OF INFECTION AGENTS IN VITRO

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TABATABAEI YAZDI FARIDEH; MORTAZAVI SEYED ALI; ALIZADEH BEHBAHANI BEHROOZ; VASIEE ALIREZA; MORADI SAMIRA; TABATABAEI YAZDI FOROUZAN; Jafarian Sara

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2015 | Volume:20 | Issue:69 Page(s): 31-40

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Title

A COMPARATIVE STUDY OF EFFECT OF TYPES OF HIBISCUS SABDARIFFA EXTRACTS AND SELECTIVE ANTIBIOTICS ON CLINICAL AND STANDARD STRAINS OF INFECTION AGENTS IN VITRO

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Keywords

HIBISCUS SABDARIFFAQ2

CLINICAL STRAINQ2

ANTIBIOTICQ3

CULTURE MEDIA CONDITIONQ2

Abstract

Background and objective: Nowadys, abundant research was done for replace of medicinal plants; plant antimicrobial components with structure of different from ANTIBIOTICs cause destroy of bacteria that are clinically important. So, the present study was carried out with purpose of antimicrobial effect investigation of different types of HIBISCUS SABDARIFFA extracts on some clinical infection agent strain and comparison with common medical ANTIBIOTICs in CULTURE MEDIA CONDITION.Materials and methods: In this study, three types of ethanolic, methanolic and aqueous extracts were prepared by method of maceration. Microbiology evaluation of strains was done using four methods: pour plate, Kirby Bauer method using disks, minimum inhibitory concentration of microbial growth (MIC) and minimum bactericidal concentration of bacteria (MBC) using Serial Dilution Method, onto six strains of infection agent. Turbidity of micro plate was determined by ELISA reading devices.Results: The ethanolic extract of HIBISCUS SABDARIFFA has a diameter of 20.16±0.50 mm in concentration of 40 mg/mL on Pseudomonas auerginisa. In the present study, the bacteria of staphylococcus aureus and Salmonella enteritidis were showed maximum and minimum sensitivity relation to aqueous extracts of HIBISCUS SABDARIFFA, respectively. Among of tested strains, Salmonella enteritidis has maximum MIC and MBC. 30 and 40 mg/mL Concentrations of Redcurrant have significant antimicrobial effect on bacteria. Antibacterial effect of extracts was decreased with decrease of extract concentration in disk.Conclusion: According to result, alcohol extracts of HIBISCUS SABDARIFFA have antimicrobial effect on growth of all of the strains exposed analyzes and antimicrobial effect of that was maximum on Gram-positive bacterum of Staphylococcus aureus.

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APA: Copy

TABATABAEI YAZDI, FARIDEH, MORTAZAVI, SEYED ALI, ALIZADEH BEHBAHANI, BEHROOZ, VASIEE, ALIREZA, MORADI, SAMIRA, TABATABAEI YAZDI, FOROUZAN, & Jafarian, Sara. (2015). A COMPARATIVE STUDY OF EFFECT OF TYPES OF HIBISCUS SABDARIFFA EXTRACTS AND SELECTIVE ANTIBIOTICS ON CLINICAL AND STANDARD STRAINS OF INFECTION AGENTS IN VITRO. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 20(69), 31-40. SID. https://sid.ir/paper/52821/en

Vancouver: Copy

TABATABAEI YAZDI FARIDEH, MORTAZAVI SEYED ALI, ALIZADEH BEHBAHANI BEHROOZ, VASIEE ALIREZA, MORADI SAMIRA, TABATABAEI YAZDI FOROUZAN, Jafarian Sara. A COMPARATIVE STUDY OF EFFECT OF TYPES OF HIBISCUS SABDARIFFA EXTRACTS AND SELECTIVE ANTIBIOTICS ON CLINICAL AND STANDARD STRAINS OF INFECTION AGENTS IN VITRO. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2015;20(69):31-40. Available from: https://sid.ir/paper/52821/en

IEEE: Copy

FARIDEH TABATABAEI YAZDI, SEYED ALI MORTAZAVI, BEHROOZ ALIZADEH BEHBAHANI, ALIREZA VASIEE, SAMIRA MORADI, FOROUZAN TABATABAEI YAZDI, and Sara Jafarian, “A COMPARATIVE STUDY OF EFFECT OF TYPES OF HIBISCUS SABDARIFFA EXTRACTS AND SELECTIVE ANTIBIOTICS ON CLINICAL AND STANDARD STRAINS OF INFECTION AGENTS IN VITRO,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 20, no. 69, pp. 31–40, 2015, [Online]. Available: https://sid.ir/paper/52821/en

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