HIGH FREQUENCY OF MIXED GENOTYPES ROTAVIRUS AMONG CHILDREN HOSPITALIZED WITH ACUTE GASTROENTERITIS. 2007-2008

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KARGAR M.; ZARE M.

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2010 | Volume:15 | Issue:49 Page(s): 1-5

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Title

HIGH FREQUENCY OF MIXED GENOTYPES ROTAVIRUS AMONG CHILDREN HOSPITALIZED WITH ACUTE GASTROENTERITIS. 2007-2008

Author(s)

KARGAR M. | ZARE M. | Issue Writer Certificate

Keywords

HUMAN ROTAVIRUSESQ3

GENOTYPINGQ2

MIXED GENOTYPESQ3

NESTED RT- PCRQ3

Abstract

Background and objective: Rotaviruses are the most important cause of severe viral gastroenteritis and dehydration diarrhea in children under five years old worldwide. This study conducted to determine genotypes of protein 7 HUMAN ROTAVIRUSES with NESTED RT- PCR among children hospitalized in Marvdasht During 2007 to 2008.Material and methods: In this study, 141 stool samples of under 5 years old hospitalized children with severe diarrhea were collected from Motahary hospital in Marvdasht. At first group A Rotaviruses were distinguished by Enzyme Immunoassay (EIA) and then, the genotypes of positive samples were determined by using 9 specific primers by Nested RT-PCR method.Results: Out of total collected samples, 40 were positive by EIA. The frequency of G1, G2 and G4 Genotypes were 5.0%, 12.5% and 10.0% respectively. G3, G8 and G9 were detected in none of specimens. The frequency of mixed and non-typeable genotypes was determind 60% and "12.5% respectively.Conclusions: Frequency of MIXED GENOTYPES indicates severe infection because of low sanitation or transmission of recombinant strains from animal to humans. Also, the noticeable frequency of non-typeable genotypes indicates the necessity of using other primers for identification of unusual genotypes.

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APA: Copy

KARGAR, M., & ZARE, M.. (2010). HIGH FREQUENCY OF MIXED GENOTYPES ROTAVIRUS AMONG CHILDREN HOSPITALIZED WITH ACUTE GASTROENTERITIS. 2007-2008. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 15(49), 1-5. SID. https://sid.ir/paper/52914/en

Vancouver: Copy

KARGAR M., ZARE M.. HIGH FREQUENCY OF MIXED GENOTYPES ROTAVIRUS AMONG CHILDREN HOSPITALIZED WITH ACUTE GASTROENTERITIS. 2007-2008. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2010;15(49):1-5. Available from: https://sid.ir/paper/52914/en

IEEE: Copy

M. KARGAR, and M. ZARE, “HIGH FREQUENCY OF MIXED GENOTYPES ROTAVIRUS AMONG CHILDREN HOSPITALIZED WITH ACUTE GASTROENTERITIS. 2007-2008,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 15, no. 49, pp. 1–5, 2010, [Online]. Available: https://sid.ir/paper/52914/en

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