PREVALENCE OF MMETHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM CLINICAL SPECIMENS OF TEACHING HOSPITALS IN MAZANDARAN USING PHENOTYPIC AND GENOTYPIC METHODS 2014-15

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MOHSENI MOJTABA; NOORPOUR NILOOFAR

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2016 | Volume:21 | Issue:73 Page(s): 45-54

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Title

PREVALENCE OF MMETHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM CLINICAL SPECIMENS OF TEACHING HOSPITALS IN MAZANDARAN USING PHENOTYPIC AND GENOTYPIC METHODS 2014-15

Author(s)

MOHSENI MOJTABA | NOORPOUR NILOOFAR | Issue Writer Certificate

Keywords

STAPHYLOCOCCUS AUREUSQ2

METHICILLIN RESISTANCEQ2

MAZANDARANQ2

Abstract

Background and objective: STAPHYLOCOCCUS AUREUS has been recognized as a major nosocomial infection. Since the 1960s, the first methicillin-resistant STAPHYLOCOCCUS AUREUS (MRSA) has appeared and resistant to all beta-lactam antibiotics caused by the mecA gene. In addition, MRSA with a wide range of infections from mild skin and soft tissue infections to life threatening pneumonia has been recently become a worldwide concern. The aim of current study was prevalence of methicillin resistant STAPHYLOCOCCUS AUREUS isolated from clinical specimens in province of MAZANDARAN using phenotypic and genotypic methods.Materials and methods: About 118 clinical specimens were collected from teaching hospitals in Babol and Sari. After identification of the isolates using usual tests, resistance to methicillin was investigated using cefoxitin disc diffusion, oxacillin screening plate and polymerase chain reaction (PCR) for mecA gene. In addition, sensitivity of isolates to other antibiotics was determined according to the Clinical and Laboratory Standards Institute (CLSI).Results: From all collected clinical specimens, a total of 100 isolates was identified as STAPHYLOCOCCUS AUREUS. The results of sensitivity assay to methicillin using cefoxitin disc diffusion and oxacillin screening agar was showed 66% and 71% of the isolates were resistant to MRSA, respectively. However, the results of PCR showed the presence of mecA gene in 81% of isolates. In addition, investigation of resistant pattern to antibiotics among the isolates was shown that the isolates were high resistant to ampicillin, penicillin and amoxicillin (100%) and low resistance to nitrofurantoin (0%). Also, the MRSA strains were resistant to at least 10 more antibiotics.Conclusion: The results of current study were shown that phenotypic methods are not reliable for determination of methicillin sensitivity. Therefore it is necessary that these results confirmed with genotypic procedures such as PCR should. Moreover, most of STAPHYLOCOCCUS AUREUS have become resistant to different antibiotics, rapid diagnosis and also appropriate and reasonable prescription of effective antibiotics in order to prevention of increasing drug resistance is necessary.

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APA: Copy

MOHSENI, MOJTABA, & NOORPOUR, NILOOFAR. (2016). PREVALENCE OF MMETHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM CLINICAL SPECIMENS OF TEACHING HOSPITALS IN MAZANDARAN USING PHENOTYPIC AND GENOTYPIC METHODS 2014-15. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 21(73), 45-54. SID. https://sid.ir/paper/52976/en

Vancouver: Copy

MOHSENI MOJTABA, NOORPOUR NILOOFAR. PREVALENCE OF MMETHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM CLINICAL SPECIMENS OF TEACHING HOSPITALS IN MAZANDARAN USING PHENOTYPIC AND GENOTYPIC METHODS 2014-15. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2016;21(73):45-54. Available from: https://sid.ir/paper/52976/en

IEEE: Copy

MOJTABA MOHSENI, and NILOOFAR NOORPOUR, “PREVALENCE OF MMETHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM CLINICAL SPECIMENS OF TEACHING HOSPITALS IN MAZANDARAN USING PHENOTYPIC AND GENOTYPIC METHODS 2014-15,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 21, no. 73, pp. 45–54, 2016, [Online]. Available: https://sid.ir/paper/52976/en

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