DETERMINATION OF CLONAL DIVERSITY OF PSEUDOMONAS AERUGINOSA ISOLATED FROM WOUND AND BURN INFECTIONS BY USING RIBOTYPING

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ASLANI M.M.; EBRAHIMIPOUR GH.; SHAHCHERAGHI F.; SHARAFI Z.; NIKBIN V.S.; HASHEMI POUR M.

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2010 | Volume:15 | Issue:50 Page(s): 45-49

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Title

DETERMINATION OF CLONAL DIVERSITY OF PSEUDOMONAS AERUGINOSA ISOLATED FROM WOUND AND BURN INFECTIONS BY USING RIBOTYPING

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Keywords

RIBOTYPINGQ3

PSEUDOMONAS AERUGINOSAQ2

WOUND AND BURN INFECTIONSQ3

Abstract

Background and objective: Determination of the relatedness of microbial isolates has become increasingly important as the number and spectrum of nosocomial pathogens continue to expand. Indeed, this approach is critical to identifying outbreaks, to determining the mode of acquisition of pathogens, and, accordingly, to defining effective preventive and therapeutic measures. The present study, was conducted to determine of the relatedness of PSEUDOMONAS AERUGINOSA isolates obtained from WOUND AND BURN INFECTIONS in Imam-khomaini, Tohid and Motahari hospitals in Tehran during 1999-2006.Material and methods: A total of 74 P. aeruginosa isolates (34 burn, 40 wound isolates) was obtained from patients during 1999-2006 of Imam-Khomeini, Tohid and Motahari hospitals in Tehran. The isolates were identified as P. aeruginosa by biochemical tests. Chromosomal DNA of the isolates was extracted by Phenol-Chloroform method and used for RIBOTYPING. RIBOTYPING was performed by using SmaI restriction enzyme.Results: 8 distinct ribotype patterns (A-H) obtained.43 (58.1%), 23 (31%) and 3 (4%) of the isolates belonged to A, B and C ribotype patterns respectively.5 (6.8%) of the isolates contained one of D-H ribotype patterns.Conclusion: RIBOTYPING indicated that the most of isolates (89%) clonally related and could be grouped into 2 major ribotype patterns. Persistence of clonal strains in these hospitals for many years indicate that is necessary to focus on reinforcement of measures for infection control.

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APA: Copy

ASLANI, M.M., EBRAHIMIPOUR, GH., SHAHCHERAGHI, F., SHARAFI, Z., NIKBIN, V.S., & HASHEMI POUR, M.. (2010). DETERMINATION OF CLONAL DIVERSITY OF PSEUDOMONAS AERUGINOSA ISOLATED FROM WOUND AND BURN INFECTIONS BY USING RIBOTYPING. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 15(50), 45-49. SID. https://sid.ir/paper/53006/en

Vancouver: Copy

ASLANI M.M., EBRAHIMIPOUR GH., SHAHCHERAGHI F., SHARAFI Z., NIKBIN V.S., HASHEMI POUR M.. DETERMINATION OF CLONAL DIVERSITY OF PSEUDOMONAS AERUGINOSA ISOLATED FROM WOUND AND BURN INFECTIONS BY USING RIBOTYPING. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2010;15(50):45-49. Available from: https://sid.ir/paper/53006/en

IEEE: Copy

M.M. ASLANI, GH. EBRAHIMIPOUR, F. SHAHCHERAGHI, Z. SHARAFI, V.S. NIKBIN, and M. HASHEMI POUR, “DETERMINATION OF CLONAL DIVERSITY OF PSEUDOMONAS AERUGINOSA ISOLATED FROM WOUND AND BURN INFECTIONS BY USING RIBOTYPING,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 15, no. 50, pp. 45–49, 2010, [Online]. Available: https://sid.ir/paper/53006/en

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