THE EFFECT OF EXTRACELLULAR MATRIX ON CHORONOTROPIC CHARACTERISTICS OF CARDIOMYOCYTES DRIVED FROM STEM CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BAHARVAND H.; AZARNIA MAHNAZ; PARIVAR KAZEM; KAZEMI ASHTIANI S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2004 | Volume:6 | Issue:23 Page(s): 181-181

Download Full-Text

View:

374

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF EXTRACELLULAR MATRIX ON CHORONOTROPIC CHARACTERISTICS OF CARDIOMYOCYTES DRIVED FROM STEM CELLS

Author(s)

BAHARVAND H. | AZARNIA MAHNAZ | PARIVAR KAZEM | KAZEMI ASHTIANI S. | Issue Writer Certificate

Keywords

EMBRYONIC STEM CELLS

CARDIOMYOCYTE DIFFERENTIATION

EXTRACELLULAR MATRIX

CHRONOTROPIC CHARACTERISTICS

Abstract

Introduction: EXTRACELLULAR MATRIX (ECM) components significantly influence the growth characteristics of cell, development of spontaneous contractile activity, andmorphologic differentiation. The present study evaluates the effect of ECM ongenotypic and physiologic characteristics of cardiomyocytes derived from embryonic stem (ES) cells.Material and Methods: Embryoid bodies derived from mouse ES cells line Royan B1 (C57BL/6) were cultured on commercial ECM (matrigel), a cardiac fibroblast-derived EXTRACELLULAR MATRIX (cardiogel), and control media (without ECM)for 21 days. The growth characteristics of cardiomyocytes were assessed by immunocytochemistery, RT-PCR, and chronotropic drugs.Results: The beating frequency of cardiomyocytes cultured on cardiogel increasedin comparison with cardiomyocytes cultured on matrigel and/or control during days 7-9(p<0.05) The spontaneously beating cells expressed markers characteristic ofcardiomyocytes including α-actinin, desmin, tropnin-I, sarcomeric myosin heavy chain (MHC), pan-cadherin, connexin 43, cardiac α-MHC, cardiacβ-MHC, myosin light chain isoform-2V, and atrial natriuretic factor. In addition,spontaneousness of cardiomyocytes on both ECMs was enhanced by treatment of cells with isopernaline, while reduced more on matrigel and carbacol when compared with control and cardiogel. However, the change in beating was similar in all groups upon treatment with phenylephrine, propranolol and for Bay-K.Conclusions: We conclude that the ECM influences ES-derived cardiomyocytes, in terms of CHRONOTROPIC CHARACTERISTICS.

Cites

No record.

References

No record.

Cite

APA: Copy

BAHARVAND, H., AZARNIA, MAHNAZ, PARIVAR, KAZEM, & KAZEMI ASHTIANI, S.. (2004). THE EFFECT OF EXTRACELLULAR MATRIX ON CHORONOTROPIC CHARACTERISTICS OF CARDIOMYOCYTES DRIVED FROM STEM CELLS. CELL JOURNAL (YAKHTEH), 6(23), 181-181. SID. https://sid.ir/paper/530161/en

Vancouver: Copy

BAHARVAND H., AZARNIA MAHNAZ, PARIVAR KAZEM, KAZEMI ASHTIANI S.. THE EFFECT OF EXTRACELLULAR MATRIX ON CHORONOTROPIC CHARACTERISTICS OF CARDIOMYOCYTES DRIVED FROM STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2004;6(23):181-181. Available from: https://sid.ir/paper/530161/en

IEEE: Copy

H. BAHARVAND, MAHNAZ AZARNIA, KAZEM PARIVAR, and S. KAZEMI ASHTIANI, “THE EFFECT OF EXTRACELLULAR MATRIX ON CHORONOTROPIC CHARACTERISTICS OF CARDIOMYOCYTES DRIVED FROM STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 6, no. 23, pp. 181–181, 2004, [Online]. Available: https://sid.ir/paper/530161/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops