USING LUMINAL SOLUTION FOR IDENTIFICATION WASHING BLOOD AND DNA TYPING

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ZANDIEH SAEID; SABAGHIAN M.; SAADATI MOJTABA

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Journal Paper

Paper Information

Journal: Iranian Journal of Forensic Medicine Year:2008 | Volume:14 | Issue:3 (51) Page(s): 143-150

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Information Journal Paper

Title

USING LUMINAL SOLUTION FOR IDENTIFICATION WASHING BLOOD AND DNA TYPING

Author(s)

ZANDIEH SAEID | SABAGHIAN M. | SAADATI MOJTABA | Issue Writer Certificate

Keywords

OCCULT BLOODQ3

LUMINOLQ3

CRIME SCENEQ3

DNA TYPINGQ3

Abstract

Background & Aim: Bloodstains can be found in most cases that a violent crime has occurred. DNA TYPING analysis can establish the genetic profile(s) of the participant in a violent crime. The recovery and detection of possible bloodstains at CRIME SCENE when a CRIME SCENE is cleaned up become even more important. Utilizing a new solution that comprises LUMINOL, it is possible to detect latent bloodstains with high sensitivity and precision.Materials & Methods: Firs, LUMINOL solution was prepared and through changing conditions and using different chemicals, the solution sensitivity was optimized, afterwards the solution examined with different blood proportions and dilutions and light intensity provided by the reaction between solution and blood was measured with the Luminometer. In addition, various surfaces and optical situations in the experiments were assessed. Eventually blood samples were used for DNA profiling.Findings: By means of the new LUMINOL solution and comparing it with the elder one, in addition to very high sensitivity to blood on various surfaces, the reaction was performed up to 10-6 dilution. Furthermore this method has no need for absolute darkness and it provides the facility for taking Photos or records without DNA damage, therefore acquiring DNA TYPING results from very small bloodstains was feasible.Conclusion: Bloodstain is one of the most important biological evidence that remains in criminal scene. In most cases criminals try to wipe it out and one of duties of the forensic group is to detect such traces. Nowadays, criminal scene is inspected using UV and also the elder LUMINOL solution that in the most cases are not sufficient. The goal of this study was to optimize and standardization of the elder LUMINOL solution and analyzing the reaction results and with distinguish between true and false results. Hence with using this method, DNA TYPING can provide judge and inspectors with powerful evidence. Some advantages of the new solution are easy to use and preparation and also it does not have any harmful effects on DNA profile determination.

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APA: Copy

ZANDIEH, SAEID, SABAGHIAN, M., & SAADATI, MOJTABA. (2008). USING LUMINAL SOLUTION FOR IDENTIFICATION WASHING BLOOD AND DNA TYPING. SCIENTIFIC JOURNAL OF FORENSIC MEDICINE, 14(3 (51)), 143-150. SID. https://sid.ir/paper/53507/en

Vancouver: Copy

ZANDIEH SAEID, SABAGHIAN M., SAADATI MOJTABA. USING LUMINAL SOLUTION FOR IDENTIFICATION WASHING BLOOD AND DNA TYPING. SCIENTIFIC JOURNAL OF FORENSIC MEDICINE[Internet]. 2008;14(3 (51)):143-150. Available from: https://sid.ir/paper/53507/en

IEEE: Copy

SAEID ZANDIEH, M. SABAGHIAN, and MOJTABA SAADATI, “USING LUMINAL SOLUTION FOR IDENTIFICATION WASHING BLOOD AND DNA TYPING,” SCIENTIFIC JOURNAL OF FORENSIC MEDICINE, vol. 14, no. 3 (51), pp. 143–150, 2008, [Online]. Available: https://sid.ir/paper/53507/en

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