EXPRESSION OF ALKALINE PHOSPHATASE DURING OSTEOGENIC DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AKBARI M.; NIKBAKHT M.; SOBHANI A.GH.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2001 | Volume:3 | Issue:11 Page(s): 131-136

Download Full-Text

View:

385

Download:

205

Cites:

Information Journal Paper

Title

EXPRESSION OF ALKALINE PHOSPHATASE DURING OSTEOGENIC DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS

Author(s)

AKBARI M. | NIKBAKHT M. | SOBHANI A.GH. | Issue Writer Certificate

Keywords

ALKALINE PHOSPHATASE

BONE MARROW

MESENCHYMAL STEM CELLS (MSCS)

OSTEOGENESIS

CULTURE

RAT

Abstract

Introduction: BONE MARROW contains a population of stem cells capable of differentiating to osteoblast and forming the bone nodule by dexamethasone. Material and Methods: The stromal cells of BONE MARROW obtained from 4 to 6 weeks old Spruge-Dawely male RATs were grown in primary CULTURE for 7 days and subcultured for 18 days. The cells were CULTUREd in either DMEM medium containing 15% fetal calf serum and antibiotics as the controls or the above medium supplemented with osteogenic supplements (OS): include 10 mM Na-β glycerophosphate (Na-βGp), 10 nM dexamethasone (Dex) and 50 g/ml ascordic acid (AsA) as the examined CULTUREs. After 6, 12 and 18 days of grow up in subculture, the CULTUREs were examined for mineralization and ALKALINE PHOSPHATASE (Apase) expression. Results: Mesenchymal stem cells (MSCs) in examined CULTUREs underwent a dramatic change in cellular morphology and a significat increase in Apase activity by day 12. The deposition of a calcified matrix on the surface of the CULTURE flasks became evident between days 12 and 18. Conclusion: The addition of osteogenic supplements (OS) to MSCs CULTUREs induced Apase expression that contributes to cellular differentiation and mineralization of extracellular matrix.

Cites

No record.

References

Cite

APA: Copy

AKBARI, M., NIKBAKHT, M., & SOBHANI, A.GH.. (2001). EXPRESSION OF ALKALINE PHOSPHATASE DURING OSTEOGENIC DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS. CELL JOURNAL (YAKHTEH), 3(11), 131-136. SID. https://sid.ir/paper/539826/en

Vancouver: Copy

AKBARI M., NIKBAKHT M., SOBHANI A.GH.. EXPRESSION OF ALKALINE PHOSPHATASE DURING OSTEOGENIC DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2001;3(11):131-136. Available from: https://sid.ir/paper/539826/en

IEEE: Copy

M. AKBARI, M. NIKBAKHT, and A.GH. SOBHANI, “EXPRESSION OF ALKALINE PHOSPHATASE DURING OSTEOGENIC DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS,” CELL JOURNAL (YAKHTEH), vol. 3, no. 11, pp. 131–136, 2001, [Online]. Available: https://sid.ir/paper/539826/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops