SYNERGISTIC EFFECT OF LPS, IFN-γ AND IRON ON APOPTOSIS OF BALB/C MICE MACROPHAGES FOLLOWING NITRIC OXIDE PRODUCTION

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PIRDEL L.; ZAVARAN HOSSEINI A.

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Journal: Iranian Journal of Basic Medical Sciences Year:2008 | Volume:11 | Issue:3 (39) Page(s): 190-196

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Title

SYNERGISTIC EFFECT OF LPS, IFN-γ AND IRON ON APOPTOSIS OF BALB/C MICE MACROPHAGES FOLLOWING NITRIC OXIDE PRODUCTION

Author(s)

PIRDEL L. | ZAVARAN HOSSEINI A. | Issue Writer Certificate

Keywords

APOPTOSIS

INTERFERON GAMMA

IRON

LIPOPOLYSACCHARIDE

MACROPHAGE

NITRIC OXIDE

Abstract

Objective(s): Previous studies have demonstrated that the NITRIC OXIDE (NO) dependent death of murine peritoneal MACROPHAGEs activated in vitro with IFN-g and LPS is mediated through APOPTOSIS. In the present study, we investigated the synergistic effect of LPS, IFN-g and IRON on NO production and APOPTOSIS.Materials and Methods: After determination of IRON cytotoxicity, the peritoneal MACROPHAGEs of Balb/c mice were cultured with IRON, LPS, and IFN-g separately, or a mixture of these for 18 hr at 37°C. Then after 18 hr incubation, the level of NO in supernatant was measured by the Griess method. At the same time, after incubation with ethidium bromide and acridine orange dye, the apoptotic MACROPHAGEs were detected by fluorescence microscopy.Results: NO production was significantly greater than the control group in MACROPHAGEs exposed to IRON, LPS, or IFN-g alone (P=0.02), while no significant difference was detected in APOPTOSIS rate in the presence of LPS (P=0.08). However, the differences were remarkable between NO production and APOPTOSIS rate in the presence of IRON, LPS and IFN-g (P£0.05).Conclusion: These findings indicate the immunostimulatory effect of IRON on NO production by IFN-g and LPS.

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APA: Copy

PIRDEL, L., & ZAVARAN HOSSEINI, A.. (2008). SYNERGISTIC EFFECT OF LPS, IFN-γ AND IRON ON APOPTOSIS OF BALB/C MICE MACROPHAGES FOLLOWING NITRIC OXIDE PRODUCTION. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 11(3 (39)), 190-196. SID. https://sid.ir/paper/543806/en

Vancouver: Copy

PIRDEL L., ZAVARAN HOSSEINI A.. SYNERGISTIC EFFECT OF LPS, IFN-γ AND IRON ON APOPTOSIS OF BALB/C MICE MACROPHAGES FOLLOWING NITRIC OXIDE PRODUCTION. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2008;11(3 (39)):190-196. Available from: https://sid.ir/paper/543806/en

IEEE: Copy

L. PIRDEL, and A. ZAVARAN HOSSEINI, “SYNERGISTIC EFFECT OF LPS, IFN-γ AND IRON ON APOPTOSIS OF BALB/C MICE MACROPHAGES FOLLOWING NITRIC OXIDE PRODUCTION,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 11, no. 3 (39), pp. 190–196, 2008, [Online]. Available: https://sid.ir/paper/543806/en

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