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Title

EFFECT OF HUMAN MENOPAUSE GONADOTROPIN AND RECOMBINANT FOLLICLE STIMULATING HORMONES ON IN VITRO MATURATION OF HUMAN IMMATURE OOCYTES AND RATE OF EIGHT CELL EMBRYOS MEDIATED BY INTRACYTOPLASMIC SPERM INJECTION

Pages

  43-50

Keywords

IVM 
HMG 
FSH 

Abstract

 Introduction: treatment of infertility by using in vitro matured oocytes derived from preovulatory follicles has many potential applications. It minimizes the risk of ovarian hypersimulation syndrome and is an alternative treatment for polycystic ovary syndrome. It also reduces costs and duration of treatment and can be important for subject who needs preservation. So provides information about final stage of oocyte maturation. We aimed to study the effects of recombinant follicle stimulating hormone (r-FSH) and human menopausal gonadotropin hormone (HMG) on human immature oocyte maturation in culture media and evaluation rate of eight-cell embryos mediated by intracytoplasmic sperm injection (ICSI). Material and methods: this experimental study was done in 2006-2007 in montasereih IVF center and mashhad Islamic azad university. One hundred fifty six immature oocytes produced by women undergoing IVF were divided to three groups in control group the oocytes were supplemented by 10% human serum albumin. In r-FSH group the oocytes were supplemented by 0.075 IU/ml r-FSH. And in the HMG group the oocytes were supplemented by 0.075 IU/ml HMG. Then matured oocytes were fertilized by ICSI and the numbers of embryos up to eight cells were investigated by invert microscope. Results: the percentage of oocytes reaching metaphase 2 and the rate of eight-cell embryos was significantly higher in the FSH and HMG groups compared with the control group (p<0.001). Conclusion: using either HMG or r-FSH can increase the rate of human immature oocytes maturation in vitro. HMG significantly increased the production rate of eight-cell embryos compared with r-FSH (p<0.001).

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    APA: Copy

    BAHARARA, J., MOUSAVIFAR, N., JALALI, M., & MAGHANI, M.. (2008). EFFECT OF HUMAN MENOPAUSE GONADOTROPIN AND RECOMBINANT FOLLICLE STIMULATING HORMONES ON IN VITRO MATURATION OF HUMAN IMMATURE OOCYTES AND RATE OF EIGHT CELL EMBRYOS MEDIATED BY INTRACYTOPLASMIC SPERM INJECTION. IRANIAN JOURNAL OF OBSTETRICS, GYNECOLOGY AND INFERTILITY, 11(3), 43-50. SID. https://sid.ir/paper/549641/en

    Vancouver: Copy

    BAHARARA J., MOUSAVIFAR N., JALALI M., MAGHANI M.. EFFECT OF HUMAN MENOPAUSE GONADOTROPIN AND RECOMBINANT FOLLICLE STIMULATING HORMONES ON IN VITRO MATURATION OF HUMAN IMMATURE OOCYTES AND RATE OF EIGHT CELL EMBRYOS MEDIATED BY INTRACYTOPLASMIC SPERM INJECTION. IRANIAN JOURNAL OF OBSTETRICS, GYNECOLOGY AND INFERTILITY[Internet]. 2008;11(3):43-50. Available from: https://sid.ir/paper/549641/en

    IEEE: Copy

    J. BAHARARA, N. MOUSAVIFAR, M. JALALI, and M. MAGHANI, “EFFECT OF HUMAN MENOPAUSE GONADOTROPIN AND RECOMBINANT FOLLICLE STIMULATING HORMONES ON IN VITRO MATURATION OF HUMAN IMMATURE OOCYTES AND RATE OF EIGHT CELL EMBRYOS MEDIATED BY INTRACYTOPLASMIC SPERM INJECTION,” IRANIAN JOURNAL OF OBSTETRICS, GYNECOLOGY AND INFERTILITY, vol. 11, no. 3, pp. 43–50, 2008, [Online]. Available: https://sid.ir/paper/549641/en

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