TRANSFORMATION OF OILSEED RAPE (B. NAPUSS L.) WITH ARABIDOPSIS 5- ENOL- PYRUVYLSHIKIMIC ACID 3-PHOSPHATE SYNTHSAE (EPSPS) GENE TOWARDS CONFERRING GLYPHOSATE RESISTANCE

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JONOUBI P.; MOUSAVI AMIR; SHEYKHANI R.; SALMANIAN ALI HATEF

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Journal Paper

Paper Information

Journal: RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE" Year:2008 | Volume:35 | Issue:6 Page(s): 59-74

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Title

TRANSFORMATION OF OILSEED RAPE (B. NAPUSS L.) WITH ARABIDOPSIS 5- ENOL- PYRUVYLSHIKIMIC ACID 3-PHOSPHATE SYNTHSAE (EPSPS) GENE TOWARDS CONFERRING GLYPHOSATE RESISTANCE

Author(s)

JONOUBI P. | MOUSAVI AMIR | SHEYKHANI R. | SALMANIAN ALI HATEF | Issue Writer Certificate

Keywords

COTYLEDONARY EXPLANTQ3

AGROBACTERUMQ3

SHIKIMATEQ3

Abstract

Glyphosate is a broad spectrum herbicide that hnhibits the 5- enol- pyruvylshikimic acid 3-phosphate synthsae (EPSPS) enzyme Which is key enzyme of SHIKIMATE pathway. One of the major approaches taken to obtain glyphosate tolerant plants is to over produce the target enzyme EPSPS. A cDNA clone encoding A. thaliana EPSPS was cloned into the pBI121 binary vector under the control of CaMV 35S promoter to abtain high level expression of the enzyme. The At EPSPS and NPT II transgenes were transformed into COTYLEDONARY EXPLANTs of rapeseed via A. tumefacience. The green transformed plantlets were obtained on selective medium suplemented with kanamicin. These plants were evaluated by PCR, DNA dot blotting and RT-PCR for confirming transgene integration and expression. T1 plants abtained from seeds of T0 transgenic plants. Phenotypic assay for glyphosate resistance and quantitative analysis of SHIKIMATE were performed after spraying the T0, T1 and wild type plants with 0, 2.5 and 5 mM glyphosate. The tesults were indicating the increased glyphosate tolerance in T0 and T1 transgenic plants.

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APA: Copy

JONOUBI, P., MOUSAVI, AMIR, SHEYKHANI, R., & SALMANIAN, ALI HATEF. (2008). TRANSFORMATION OF OILSEED RAPE (B. NAPUSS L.) WITH ARABIDOPSIS 5- ENOL- PYRUVYLSHIKIMIC ACID 3-PHOSPHATE SYNTHSAE (EPSPS) GENE TOWARDS CONFERRING GLYPHOSATE RESISTANCE. RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE", 35(6), 59-74. SID. https://sid.ir/paper/55925/en

Vancouver: Copy

JONOUBI P., MOUSAVI AMIR, SHEYKHANI R., SALMANIAN ALI HATEF. TRANSFORMATION OF OILSEED RAPE (B. NAPUSS L.) WITH ARABIDOPSIS 5- ENOL- PYRUVYLSHIKIMIC ACID 3-PHOSPHATE SYNTHSAE (EPSPS) GENE TOWARDS CONFERRING GLYPHOSATE RESISTANCE. RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE"[Internet]. 2008;35(6):59-74. Available from: https://sid.ir/paper/55925/en

IEEE: Copy

P. JONOUBI, AMIR MOUSAVI, R. SHEYKHANI, and ALI HATEF SALMANIAN, “TRANSFORMATION OF OILSEED RAPE (B. NAPUSS L.) WITH ARABIDOPSIS 5- ENOL- PYRUVYLSHIKIMIC ACID 3-PHOSPHATE SYNTHSAE (EPSPS) GENE TOWARDS CONFERRING GLYPHOSATE RESISTANCE,” RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE", vol. 35, no. 6, pp. 59–74, 2008, [Online]. Available: https://sid.ir/paper/55925/en

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