EFFECTS OF SAFFRON (CROCUS SATIVUS L.) AQUEOUS EXTRACT ON IN VITRO MATURATION, FERTILIZATION AND EMBRYO DEVELOPMENT OF MOUSE OOCYTES

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TAVANA SOMAYEH; EIMANI HOSSEIN; AZARNIA MAHNAZ; SHAHVERDI ABDOLHOSSEIN; EFTEKHARI YAZDI POOPAK

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Journal: Cell Journal (Yakhteh) Year:2012 | Volume:13 | Issue:4 (52) Page(s): 259-264

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Title

EFFECTS OF SAFFRON (CROCUS SATIVUS L.) AQUEOUS EXTRACT ON IN VITRO MATURATION, FERTILIZATION AND EMBRYO DEVELOPMENT OF MOUSE OOCYTES

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Keywords

CROCUS SATIVUS

OOCYTE

IN VITRO MATURATION

ANTIOXIDANT

MICE

Abstract

Objective: Lower pregnancy rates of in vitro matured OOCYTEs compared to those of in vivo stimulated cycles indicate that optimization of IN VITRO MATURATION (IVM) remains a challenge. Reduced developmental competence of in vitro matured OOCYTEs shows that current culture systems for OOCYTE maturation do not adequately support nuclear and/or cytoplasmic maturation. Therefore this study evaluates the effects of different concentrations of saffron (CROCUS SATIVUS L.) aqueous extract (SAE), as an ANTIOXIDANT agent on IVM of immature mouse OOCYTEs.Materials and Methods: In this experimental study, cumulus-oocyte complexes (COCs) were collected from 6-8 weeks old novel medical research institute (NMRI) female MICE ovaries. COCs were cultured in IVM medium supplemented with 0 (control), 5, 10, 20 and 40mg/ml of SAE in 5% CO2 at 37oC. The rates of maturation, fertilization and development were recorded. ANOVA and Duncan’s protected least significant test, using the SAS program was applied for all statistical analysis.Results: The maturation rate was significantly higher in all groups treated with different concentrations of SAE compared with the control group (p<0.05). However, the lower concentrations of SAE (10 and 5mg/ml) in maturation medium respectively increased the fertilization rate of OOCYTEs and in vitro developmental competence when compared with the control group (p<0.05).Conclusion: The results of this study indicate that lower concentrations of SAE are more appropriate to be added to maturation medium when compared with other experimental and control groups. Generally, we conclude that addition of appropriate amounts of natural extracts such as SAE to maturation medium improves OOCYTE maturation and embryo development.

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APA: Copy

TAVANA, SOMAYEH, EIMANI, HOSSEIN, AZARNIA, MAHNAZ, SHAHVERDI, ABDOLHOSSEIN, & EFTEKHARI YAZDI, POOPAK. (2012). EFFECTS OF SAFFRON (CROCUS SATIVUS L.) AQUEOUS EXTRACT ON IN VITRO MATURATION, FERTILIZATION AND EMBRYO DEVELOPMENT OF MOUSE OOCYTES. CELL JOURNAL (YAKHTEH), 13(4 (52)), 259-264. SID. https://sid.ir/paper/565909/en

Vancouver: Copy

TAVANA SOMAYEH, EIMANI HOSSEIN, AZARNIA MAHNAZ, SHAHVERDI ABDOLHOSSEIN, EFTEKHARI YAZDI POOPAK. EFFECTS OF SAFFRON (CROCUS SATIVUS L.) AQUEOUS EXTRACT ON IN VITRO MATURATION, FERTILIZATION AND EMBRYO DEVELOPMENT OF MOUSE OOCYTES. CELL JOURNAL (YAKHTEH)[Internet]. 2012;13(4 (52)):259-264. Available from: https://sid.ir/paper/565909/en

IEEE: Copy

SOMAYEH TAVANA, HOSSEIN EIMANI, MAHNAZ AZARNIA, ABDOLHOSSEIN SHAHVERDI, and POOPAK EFTEKHARI YAZDI, “EFFECTS OF SAFFRON (CROCUS SATIVUS L.) AQUEOUS EXTRACT ON IN VITRO MATURATION, FERTILIZATION AND EMBRYO DEVELOPMENT OF MOUSE OOCYTES,” CELL JOURNAL (YAKHTEH), vol. 13, no. 4 (52), pp. 259–264, 2012, [Online]. Available: https://sid.ir/paper/565909/en

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