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Information Journal Paper

Title

DETECTION OF CTX-M-β LACTAMASES IN ISOLATED KLEBSIELLA PNEUMONIAE

Pages

  137-142

Abstract

 Background and Objective: Organisms producing CTX-M b-lactamases are emerging as a source of resistance to oxyiminocephalosporins such as ceftriaxone and ceftazidime. However, the laboratory detection of these strains is not well defined. In this study, phenotypic assay for screening of extended-spectrum b-lactamases producing strains and molecular assay for the identification of CTX-M b-lactamases genes was developed and used to investigate the prevalence of these enzymes among clinical isolates of KLEBSIELLA PNEUMONIAE in three general hospitals of Tehran, Iran. Materials and Methods: Phenotypic detection was used for screening of isolates by agar dilution method. A decrease of ³3 doubling dilution in an MIC for either ceftriaxone or ceftazidime tested in combination with 4 mg/l clavulanic acid (prepared from Glasco Smith company) versus its MIC when tested alone, confirmed an ESBL-producing organism. The PCR assay consisted of four primer sets. Results: In initial screening test, 117 (69%) from 168 clinical isolates were positive and 51 isolates (31%) were negative. From the positive isolates, 96 isolates were positive in phenotypic confirmatory test. Using molecular assay, 117 strains potentially producing extended-spectrum-b-lactamases were examined for the presence of CTX-M enzymes. 88 strains (75.2%) were positive for blactx-m group I genes, 1 strain (0.85%) was positive for blactx-m group III greens, and 2 strains (1.7%) were positive for blactx-m group IV.Conclusion: The prevalence of extended-spectrum b-lactamases (ESBLs) are increasing significantly in hospitals of Tehran. In other side, we found that the CTX-M I group had the most prevalence than other CTX-M groups.

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    Cite

    APA: Copy

    BAMERI, Z., CHITSAZ, MOHSEN, & OULIA, P.. (2010). DETECTION OF CTX-M-β LACTAMASES IN ISOLATED KLEBSIELLA PNEUMONIAE. IRANIAN JOURNAL OF PATHOLOGY (IJP), 5(3), 137-142. SID. https://sid.ir/paper/569086/en

    Vancouver: Copy

    BAMERI Z., CHITSAZ MOHSEN, OULIA P.. DETECTION OF CTX-M-β LACTAMASES IN ISOLATED KLEBSIELLA PNEUMONIAE. IRANIAN JOURNAL OF PATHOLOGY (IJP)[Internet]. 2010;5(3):137-142. Available from: https://sid.ir/paper/569086/en

    IEEE: Copy

    Z. BAMERI, MOHSEN CHITSAZ, and P. OULIA, “DETECTION OF CTX-M-β LACTAMASES IN ISOLATED KLEBSIELLA PNEUMONIAE,” IRANIAN JOURNAL OF PATHOLOGY (IJP), vol. 5, no. 3, pp. 137–142, 2010, [Online]. Available: https://sid.ir/paper/569086/en

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