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Information Journal Paper

Title

IMMUNOLOCALIZATION OF GALECTIN-3 IN MOUSE TESTICULAR TISSUE

Pages

  349-353

Keywords

Abstract

 Objective (s): GALECTIN-3 (Gal-3) is a member of the b-galactoside-binding LECTINs which is expressed in a variety of tissues and plays a role in diverse biological events, such as embryogenesis, adhesion, cellular proliferation, and apoptosis. In this study, the presence and distribution of GALECTIN-3 (Gal-3) in the mouse testicular tissue was investigated.Materials and Methods: Eight adult NMRI MICE were used in this study. Animals were sacrificed by decapitation under ether anesthesia. The testes were excised, fixed in 10% buffered formalin and embedded in paraffin for immunohistochemical (IHC) studies. H-score, a semi-quantitative method, was used for scaling the immunostaining.Results: Positive immunoreactivity to Gal-3 was detected in the connective tissues of the interstitium, Leydig cells and presumably peritubular myoid cells. Seminiferous tubules showed immuno-positive reaction in a stage dependent manner. Stages I-III and IX-XII of spermatogenic cycle showed mild immunostaining, while moderate immunostaining was observed at stages IV-VI. Highest immunoreactivity was observed at stages VII-VIII. H-score assessment showed a significant increase in stages of VII-VIII in comparison to other stages (P<0.05).Conclusion: Expression of Gal-3 in interstitial tissue and seminiferous tubules indicated that this protein probably has multifunctional roles in the mouse testicular tissue.

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    APA: Copy

    KHORSANDI, LAYASADAT, & ORAZIZADEH, MAHMOUD. (2011). IMMUNOLOCALIZATION OF GALECTIN-3 IN MOUSE TESTICULAR TISSUE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 14(4 (51)), 349-353. SID. https://sid.ir/paper/569685/en

    Vancouver: Copy

    KHORSANDI LAYASADAT, ORAZIZADEH MAHMOUD. IMMUNOLOCALIZATION OF GALECTIN-3 IN MOUSE TESTICULAR TISSUE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2011;14(4 (51)):349-353. Available from: https://sid.ir/paper/569685/en

    IEEE: Copy

    LAYASADAT KHORSANDI, and MAHMOUD ORAZIZADEH, “IMMUNOLOCALIZATION OF GALECTIN-3 IN MOUSE TESTICULAR TISSUE,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 14, no. 4 (51), pp. 349–353, 2011, [Online]. Available: https://sid.ir/paper/569685/en

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