مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

316
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

154
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

CLONING AND EXPRESSION ANALYSIS OF TWO PHOTOSYNTHETIC GENES, PSI-H AND LHCB1, UNDER TREHALOSE FEEDING CONDITIONS IN ARABIDIPSIS SEEDLINGS

Pages

  179-187

Abstract

 Trehalose (a-D-glucosyl-[1,1]- a-D-glucopyranoside) is involved in mechanisms that coordinate metabolism with plant growth adaptation and development. The main objective of the current work was to find out whether trehalose feeding affects the expression of two genes involved in photosynthesis: one gene coding for photosystem1 subunit H (PS1-H) and the other for the light harvesting complex B1 (LHCB1). In this study, Arabidopsis seeds were grown on one-halfstrength medium supplemented with 100 mM trehalose or sorbitol (as osmotic control) for 2 weeks. Trehalose-fed seedlings showed inhibited root growth, delayed emergence of primary cotyledons and darkrimmed cotyledons. Exogenously applied trehalose strongly induced starch accumulation in cotyledons and, concomitantly, depletion of starch in collumella cells of the root cap. Gene expression analysis of photosynthetic genes in Arabidopsis seedlings revealed that trehalose feeding repressed PS1-H and LHCB1 expression as compared to sorbitol-fed seedlings. To confirm trehalose inhibitory effect on photosynthetic gene expression, the cDNA of PS1-H and LHCB1 was used to transform Arabidopsis seedlings. Transformed lines showed higher transcript levels of PS1-H and LHCB1.Trehalose feeding also reduced expression levels of PS1-H and LHCB1 in transformed lines.These findings show that trehalose down-regulates the expression of two genes encoding typical components of the photosynthetic machinery.

Multimedia

  • No record.

    • Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    AGHDASI, MAHNAZ, & SCHLUEPMAN, H.. (2009). CLONING AND EXPRESSION ANALYSIS OF TWO PHOTOSYNTHETIC GENES, PSI-H AND LHCB1, UNDER TREHALOSE FEEDING CONDITIONS IN ARABIDIPSIS SEEDLINGS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 7(3), 179-187. SID. https://sid.ir/paper/573321/en

    Vancouver: Copy

    AGHDASI MAHNAZ, SCHLUEPMAN H.. CLONING AND EXPRESSION ANALYSIS OF TWO PHOTOSYNTHETIC GENES, PSI-H AND LHCB1, UNDER TREHALOSE FEEDING CONDITIONS IN ARABIDIPSIS SEEDLINGS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2009;7(3):179-187. Available from: https://sid.ir/paper/573321/en

    IEEE: Copy

    MAHNAZ AGHDASI, and H. SCHLUEPMAN, “CLONING AND EXPRESSION ANALYSIS OF TWO PHOTOSYNTHETIC GENES, PSI-H AND LHCB1, UNDER TREHALOSE FEEDING CONDITIONS IN ARABIDIPSIS SEEDLINGS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 7, no. 3, pp. 179–187, 2009, [Online]. Available: https://sid.ir/paper/573321/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button