مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

COMPARISON OF THE PHOTOBLEACHING AND PHOTOSTABILITY TRAITS OF ALEXA FLUOR 568- AND FLUORESCEIN ISOTHIOCYANATE- CONJUGATED ANTIBODY

Pages

  169-172

Abstract

 Objective: Synthetic fluorescent dyes that are conjugated to antibodies are useful tools to probe molecules. Based on dye chemical structures, their PHOTOBLEACHING and PHOTOSTABILITY indices are quite diverse. It is generally believed that among different fluorescent dyes, Alexa Fluor family has greater PHOTOSTABILITY than traditional dyes like FLUORESCEIN ISOTHIOCYANATE (FITC) and Cy5. ALEXA FLUOR 568 is a member of Alexa Fluor family presumed to have superior PHOTOSTABILITY and photobleahing profiles than FITC.Materials and Methods: In this experimental study, we conjugated ALEXA FLUOR 568 and FITC dyes to a mouse anti-human nestin monoclonal antibody (ANM) to acquire their PHOTOBLEACHING profiles and PHOTOSTABILITY indices. Then, the fluorophore/antibody ratios were calculated using a spectrophotometer. The PHOTOBLEACHING profiles and PHOTOSTABILITY indices of conjugated antibodies were subsequently studied by immunocytochemistry (ICC). Samples were continuously illuminated and digital images acquired under a fluorescent microscope. Data were processed by ImageJ software.Results: ALEXA FLUOR 568 has a brighter fluorescence and higher PHOTOSTABILITY than FITC.Conclusion: ALEXA FLUOR 568 is a capable dye to use in photostaining techniques and it has a longer PHOTOSTABILITY when compared to FITC.

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    APA: Copy

    MAHMOUDIAN, JAFAR, HADAVI, REZA, JEDDI TEHRANI, MAHMOOD, MAHMOUDI, AHMAD REZA, BAYAT, ALI AHMAD, SHABAN, ELHAM, VAFAKHAH, MOHTARAM, DARZI, MARYAM, TARAHOMI, MAJID, & GHODS, ROYA. (2011). COMPARISON OF THE PHOTOBLEACHING AND PHOTOSTABILITY TRAITS OF ALEXA FLUOR 568- AND FLUORESCEIN ISOTHIOCYANATE- CONJUGATED ANTIBODY. CELL JOURNAL (YAKHTEH), 13(3 (51)), 169-172. SID. https://sid.ir/paper/574209/en

    Vancouver: Copy

    MAHMOUDIAN JAFAR, HADAVI REZA, JEDDI TEHRANI MAHMOOD, MAHMOUDI AHMAD REZA, BAYAT ALI AHMAD, SHABAN ELHAM, VAFAKHAH MOHTARAM, DARZI MARYAM, TARAHOMI MAJID, GHODS ROYA. COMPARISON OF THE PHOTOBLEACHING AND PHOTOSTABILITY TRAITS OF ALEXA FLUOR 568- AND FLUORESCEIN ISOTHIOCYANATE- CONJUGATED ANTIBODY. CELL JOURNAL (YAKHTEH)[Internet]. 2011;13(3 (51)):169-172. Available from: https://sid.ir/paper/574209/en

    IEEE: Copy

    JAFAR MAHMOUDIAN, REZA HADAVI, MAHMOOD JEDDI TEHRANI, AHMAD REZA MAHMOUDI, ALI AHMAD BAYAT, ELHAM SHABAN, MOHTARAM VAFAKHAH, MARYAM DARZI, MAJID TARAHOMI, and ROYA GHODS, “COMPARISON OF THE PHOTOBLEACHING AND PHOTOSTABILITY TRAITS OF ALEXA FLUOR 568- AND FLUORESCEIN ISOTHIOCYANATE- CONJUGATED ANTIBODY,” CELL JOURNAL (YAKHTEH), vol. 13, no. 3 (51), pp. 169–172, 2011, [Online]. Available: https://sid.ir/paper/574209/en

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