Study of integrons class 1, 2, and 3 frequencies in Salmonella Typhimurium isolated from clinical samples using polymerase chain reaction

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOGHADAM A.; NAZARIAN S.; OLAD GH.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Shahrekord University of Medical Sciences Year:2019 | Volume:20 | Issue:5 Page(s): 80-90

Download Full-Text

Persian Verion

View:

366

Download:

Cites:

Information Journal Paper

Title

Study of integrons class 1, 2, and 3 frequencies in Salmonella Typhimurium isolated from clinical samples using polymerase chain reaction

Author(s)

MOGHADAM A. | NAZARIAN S. | OLAD GH.R. | Issue Writer Certificate

Keywords

Salmonella typhimuriumQ2

Drug resistanceQ2

IntegronQ1

Abstract

Background and aims: Presence of Integrons class 1, 2, and 3 in Salmonella typhimurium with multiDrug resistance have been raised concerns about the Integrons’ frequencies in clinic. Regarding different drug resistant genes are located in Integrons and on the other hand, Integrons are able to spread rapidly, so that identification of isolates carrying Integrons could provide useful information about the degree of resistance. The gaim of this study was the molecular analysis of Integrons class 1, 2 and 3 as important Integron classes in Salmonella typhimurium strains which are isolated from patients by using multiplex PCR. Methods: In this study, 891 samples are collected from patients with acute gastroenteritis and then Salmonella typhimurium strains were detected by using standard biochemical test and PCR in culture method. Antimicrobial susceptibility test was done on Mü ller-Hinton agar media according to the Clinical and laboratory standards institute (CLSI) guidelines. After DNA extraction, the presence of Integrons class 1, 2, and 3 were analyzed by multiplex PCR. Results: Out of 891 samples, 48 strains detected as a Salmonella typhimurium. Out of these 48 strains, 46 strains (95. 8%) had the intI gene, 2 strains (4. 1%) had the intII gene, and 18 strains (37. 5%) had the intIII gene. Antimicrobial susceptibility test showed that the most antibiotic resistance pattern was observed to cefotaxime. Strians with integrin genes had resistance to Gentamicin, Ampicillin, Tetracycline, Co-amoxiclave and Trimethoprim antibiotics. Conclusion: In this study, we observed the high incidence of Integrons class 1, 2, and 3 in comparison to previous studies which were presumably due to differences in hygiene and also overuse of antibiotics. Carrying out appropriate methods for controlling infection and treatment in studied health centers are essential for preventing the further spread of multi drug resistant Salmonella typhimurium.

Cites

No record.

References

No record.

Cite

APA: Copy

MOGHADAM, A., NAZARIAN, S., & OLAD, GH.R.. (2019). Study of integrons class 1, 2, and 3 frequencies in Salmonella Typhimurium isolated from clinical samples using polymerase chain reaction. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, 20(5 ), 80-90. SID. https://sid.ir/paper/58063/en

Vancouver: Copy

MOGHADAM A., NAZARIAN S., OLAD GH.R.. Study of integrons class 1, 2, and 3 frequencies in Salmonella Typhimurium isolated from clinical samples using polymerase chain reaction. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;20(5 ):80-90. Available from: https://sid.ir/paper/58063/en

IEEE: Copy

A. MOGHADAM, S. NAZARIAN, and GH.R. OLAD, “Study of integrons class 1, 2, and 3 frequencies in Salmonella Typhimurium isolated from clinical samples using polymerase chain reaction,” JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, vol. 20, no. 5 , pp. 80–90, 2019, [Online]. Available: https://sid.ir/paper/58063/en

Related Journal Papers