COULD MDMA PROMOTE STEMNESS CHARACTERISTICS IN MOUSE EMBRYONIC STEM CELLS VIA MGLU5 METABOTROPIC GLUTAMATE RECEPTORS?

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MEAMAR ROKHSAREH; KARAMALI FERESHTEH; MOUSAVI SEYED ALI; BAHARVAND HOSSEIN; NASRESFAHANI MOHAMMAD HOSSEIN

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Journal: Cell Journal (Yakhteh) Year:2012 | Volume:14 | Issue:3 (55) Page(s): 185-192

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Title

COULD MDMA PROMOTE STEMNESS CHARACTERISTICS IN MOUSE EMBRYONIC STEM CELLS VIA MGLU5 METABOTROPIC GLUTAMATE RECEPTORS?

Author(s)

Keywords

EMBRYONIC STEM CELLS

ECSTASY

MDMA

SELF RENEWAL

MGLU RECEPTOR

Abstract

Objective: ECSTASY, or 3, 4(±) methylenedioxymethamphetamine (MDMA), is a potent neurotoxic drug.One of the mechanisms for its toxicity is the secondary release of glutamate.Mouse EMBRYONIC STEM CELLS (mESCs) express only one glutamate receptor, the metabotropic glutamate receptor 5 (mGlu5), which is involved in the maintenance and self-renewal of mESCs. This study aims to investigate whether MDMA could influence self-renewal via the mGlu5 receptor in mESCs.Materials and Methods: In this experimental study, we used immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR) to determine the presence of the mGlu5 receptor in mESCs. The expression of mGlu5 was evaluated after MDMA was added to mESCs throughout neural precursor cell formation as group 1 and during neural precursor cell differentiation as group 2. The stemness characteristic in treated mESCs by immunofluorescence and flow cytometry was studied. Finally, caspase activity was evaluated by fluorescence staining in the treated group. One-way ANOVA or repeated measure of ANOVA according to the experimental design was used for statistical analyses.Results: In this study mGlu5 expression was shown in mESCs. In terms of neuronal differentiation, MDMA affected mGlu5 expression during neural precursor cell formation (group 1) and not during neural precursor differentiation (group 2). MDMA (450 mM) induced a significant increment in self-renewal properties in mESCs but did not reverse 2-methyl-6 (phenylethynyl) pyridine (MPEP, 1mM), a non-competitive selective mGlu5 antagonist.Fluorescence staining with anti-caspase 3 showed a signifi cant increase in the number of apoptotic cells in the MDMA group.Conclusion: We observed a dual role for MDMA on mESCs: reduced proliferation and maintenance of self-renewal. The lack of decreasing stemness characteristic in presence of MPEP suggests that MDMA mediates its role through a different mechanism that requires further investigation. In conclusion, despite being toxic, MDMA maintains stemness characteristics.

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APA: Copy

MEAMAR, ROKHSAREH, KARAMALI, FERESHTEH, MOUSAVI, SEYED ALI, BAHARVAND, HOSSEIN, & NASRESFAHANI, MOHAMMAD HOSSEIN. (2012). COULD MDMA PROMOTE STEMNESS CHARACTERISTICS IN MOUSE EMBRYONIC STEM CELLS VIA MGLU5 METABOTROPIC GLUTAMATE RECEPTORS?. CELL JOURNAL (YAKHTEH), 14(3 (55)), 185-192. SID. https://sid.ir/paper/582067/en

Vancouver: Copy

MEAMAR ROKHSAREH, KARAMALI FERESHTEH, MOUSAVI SEYED ALI, BAHARVAND HOSSEIN, NASRESFAHANI MOHAMMAD HOSSEIN. COULD MDMA PROMOTE STEMNESS CHARACTERISTICS IN MOUSE EMBRYONIC STEM CELLS VIA MGLU5 METABOTROPIC GLUTAMATE RECEPTORS?. CELL JOURNAL (YAKHTEH)[Internet]. 2012;14(3 (55)):185-192. Available from: https://sid.ir/paper/582067/en

IEEE: Copy

ROKHSAREH MEAMAR, FERESHTEH KARAMALI, SEYED ALI MOUSAVI, HOSSEIN BAHARVAND, and MOHAMMAD HOSSEIN NASRESFAHANI, “COULD MDMA PROMOTE STEMNESS CHARACTERISTICS IN MOUSE EMBRYONIC STEM CELLS VIA MGLU5 METABOTROPIC GLUTAMATE RECEPTORS?,” CELL JOURNAL (YAKHTEH), vol. 14, no. 3 (55), pp. 185–192, 2012, [Online]. Available: https://sid.ir/paper/582067/en

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