THE EFFECTS OF NIGELLA SATIVA ETHANOLIC EXTRACT ON PROLIFERATION AND APOPTOSIS OF RENAL CELL CARCINOMA ACHN CELL LINE

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TABASI N.A.S.; KHAJAVIRAD A.A.F.; MAHMOUDI MAHMOUD; BAHARARA J.; RASTIN M.; HOSSEINPOUR MASHHADI M.; SHEYKH AKRAM; ZAMANI TAGHIZADEH RABE SH.; AGHAEI AZITA; AHADI B.B.M.

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Journal: Journal of Shahrekord University of Medical Sciences Year:2010 | Volume:12 | Issue:3 Page(s): 7-14

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Title

THE EFFECTS OF NIGELLA SATIVA ETHANOLIC EXTRACT ON PROLIFERATION AND APOPTOSIS OF RENAL CELL CARCINOMA ACHN CELL LINE

Author(s)

Keywords

APOPTOTICQ3

ACHN CELL LINEQ3

CYTOTOXICQ3

ETHANOLIC EXTRACTQ2

FLOW CYTOMETRYQ3

L929 CELL LINEQ2

NIGELLA SATIVAQ3

Abstract

Background and aims: Black seed (Nigella sativa), belongs to Rannunculaceae family. It has various functions, including immuno-modulatory, anti-oxidant, anti-inflammatory and anti-neoplastic effects. Anticancer properties of NS compounds are related to their CYTOTOXIC and APOPTOTIC effects. In this study, CYTOTOXIC and APOPTOTIC effects of ethanol: extract of NS was investigated on human kidney cancer cell line (ACHN) and normal cell line (L929).Methods: NS ETHANOLIC EXTRACT at 0, 10, 50, 100, 250, 500, 750, 1000, 1250, 1500, 1750 and 2000µg/ml concentrations were prepared and studied in culture mediums of ACHN and L929 cells. After 24, 48 and 72h, morphologic changes and MTT assay were performed in both cell lines. Indeed, early and late apoptosis were studied by phosphatidyl serine kit employing FLOW CYTOMETRY technique.Results: After 24h, NS extract at 750 mg/ml and higher concentrations caused morphological changes and also reduced percentage of alive cells significantly (P<0.05) in ACHN cells compared with L929 cells that showed similar changes at 1250 mg/ml and higher concentrations. After 48 and 72h, these effects were more prominent. MTT assay results, showed that NS concentration of 750mg/ml and higher doses reduce percentage of alive ACHN cells (P<0.05). L929 cells showed similar changes at 1250mg/ml and higher concentrations (P<0.05). Maximum apoptosis in ACHN cells was happened at 1000 & 1250mg/ml (92%) compared with L929 cells that showed maximum apoptosis at 1500mg/ml (89%). Indeed, both cell lines showed a significant increase in apoptosis compared with their control groups (P<0.001).Conclusion: NS ETHANOLIC EXTRACT can may be useful as a CYTOTOXIC and chemotherapeutic agent in kidney cancer treatment.

Cites

THE EFFECT OF NIGELLA SATIVA ON THE ACTIVITY OF DRUGS-METABOLIZING ENZYMES AND BETA CATENIN LEVEL IN RAT TREATED WITH DIMETHYLHYDRAZINE

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APA: Copy

TABASI, N.A.S., KHAJAVIRAD, A.A.F., MAHMOUDI, MAHMOUD, BAHARARA, J., RASTIN, M., HOSSEINPOUR MASHHADI, M., SHEYKH, AKRAM, ZAMANI TAGHIZADEH RABE, SH., AGHAEI, AZITA, & AHADI, B.B.M.. (2010). THE EFFECTS OF NIGELLA SATIVA ETHANOLIC EXTRACT ON PROLIFERATION AND APOPTOSIS OF RENAL CELL CARCINOMA ACHN CELL LINE. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, 12(3), 7-14. SID. https://sid.ir/paper/58331/en

Vancouver: Copy

TABASI N.A.S., KHAJAVIRAD A.A.F., MAHMOUDI MAHMOUD, BAHARARA J., RASTIN M., HOSSEINPOUR MASHHADI M., SHEYKH AKRAM, ZAMANI TAGHIZADEH RABE SH., AGHAEI AZITA, AHADI B.B.M.. THE EFFECTS OF NIGELLA SATIVA ETHANOLIC EXTRACT ON PROLIFERATION AND APOPTOSIS OF RENAL CELL CARCINOMA ACHN CELL LINE. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2010;12(3):7-14. Available from: https://sid.ir/paper/58331/en

IEEE: Copy

N.A.S. TABASI, A.A.F. KHAJAVIRAD, MAHMOUD MAHMOUDI, J. BAHARARA, M. RASTIN, M. HOSSEINPOUR MASHHADI, AKRAM SHEYKH, SH. ZAMANI TAGHIZADEH RABE, AZITA AGHAEI, and B.B.M. AHADI, “THE EFFECTS OF NIGELLA SATIVA ETHANOLIC EXTRACT ON PROLIFERATION AND APOPTOSIS OF RENAL CELL CARCINOMA ACHN CELL LINE,” JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, vol. 12, no. 3, pp. 7–14, 2010, [Online]. Available: https://sid.ir/paper/58331/en

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