MICRORNA-143 INHIBITS MIGRATION OF HUMAN PROSTATE CANCER CELLS (PC3)

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BAZHAN E.; BARADARAN B.

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Journal: JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS) Year:2017 | Volume:17 | Issue:2 Page(s): 142-153

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Information Journal Paper

Title

MICRORNA-143 INHIBITS MIGRATION OF HUMAN PROSTATE CANCER CELLS (PC3)

Author(s)

BAZHAN E. | BARADARAN B. | Issue Writer Certificate

Keywords

MIRNA-143Q1

PROSTATE CANCERQ2

METASTASISQ2

TRANSFECTIONQ1

Abstract

Background & objectives: PROSTATE CANCER is one the most common cancer in men whose incidence is increasing in many countries. According to the studies, decreased expression of miR-143 has been reported in PROSTATE CANCER. In this study, we replaced MIRNA-143 in PROSTATE CANCER cells by vector based MIRNA-143 and evaluated its inhibitory effects on migration of PROSTATE CANCER cells (PC3).Methods: MTT assay was performed to reach an inhibitory concentration of Genticin antibiotic (G418(in PC3 cells. Then, MIRNA-143 vector was transfected into PC3 cells via JetPEI TRANSFECTION reagent. The transfected cells were selected by G418 antibiotic according to a 2-week treatment with IC50 concentration. Then, the expression level of MIRNA-143 was measured by qRT-PCR method. To evaluate the effect of MIRNA-143 in inhibition of migration, scratch wound healing assay was performed.Results: Results of MTT assay showed the IC50 level of G418 on PC3 cells was obtained to be 141.9 mg / ml. The results of qRT-PCR indicated increased expression of MIRNA-143 in PC3 cells transfected with MIRNA-143 compared to control cells. Finally, the results of wound healing assay showed migration reduction in transfected cells compared with control cells (empty vector).Conclusion: The results showed that MIRNA-143 play an important role in cell migration during PROSTATE CANCER METASTASIS, and it can be a good candidate for molecular treatments.

Cites

Evaluation of gene expression level of GPRC6A, E. cadherin and ZEB1 in prostate cancer in comparison to benign tissues

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APA: Copy

BAZHAN, E., & BARADARAN, B.. (2017). MICRORNA-143 INHIBITS MIGRATION OF HUMAN PROSTATE CANCER CELLS (PC3). JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 17(2), 142-153. SID. https://sid.ir/paper/59609/en

Vancouver: Copy

BAZHAN E., BARADARAN B.. MICRORNA-143 INHIBITS MIGRATION OF HUMAN PROSTATE CANCER CELLS (PC3). JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2017;17(2):142-153. Available from: https://sid.ir/paper/59609/en

IEEE: Copy

E. BAZHAN, and B. BARADARAN, “MICRORNA-143 INHIBITS MIGRATION OF HUMAN PROSTATE CANCER CELLS (PC3),” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 17, no. 2, pp. 142–153, 2017, [Online]. Available: https://sid.ir/paper/59609/en

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