Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

401
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

341
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

DETERMINING INFLUENZA VIRUS SHEDDING AT DIFFERENT TIME POINTS IN MADIN-DARBY CANINE KIDNEY CELL LINE

Pages

  130-135

Abstract

 Objective: Monitoring of INFLUENZA VIRUS shedding and optimization of multiplicities of infection (MOI) is important in the investigation of a virus one step growth cycle and for obtaining a high yield of virus in vaccine development and conventional basic diagnostic methods. However, eluted infectious viruses may still be present immediately after virus inoculation and when cells are washed following virus cultivation which may lead to a false positive virus infectivity assay.Materials and Methods: In this experimental study, we investigated INFLUENZA VIRUS progeny production in Madin-Darby canine kidney (MDCK) cells with five different MOI at determined time points. The results were analyzed by end point titration tests and immunofluorescence assay.Results: Higher titers of eluted virus were observed following a high MOI inoculation of virus in cell culture. Most probably, this was the result of sialic acid residues from viral hemagglutin in proteins that were cleaved by neuraminidase glycoproteins on the surface of the INFLUENZA VIRUS, which promoted viral spread from the host cell to the culture supernatant or during endocytosis, where viruses recycle to the cell surface by recycling ENDOSOMEs which culminated in VIRUS SHEDDING without replication.Conclusion: We demonstrated that the pattern of INFLUENZA VIRUS progeny production was dose-dependent and not uniform. This production was influenced by several factors, particularly MOI. Understanding the exact features of viral particle propagation has a major impact in producing high virus yields in the development of vaccines. Use of lower MOI (0.01) could result in accurate, precise quantitative assays in virus diagnosis and titration methods.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    ABDOLI, ASGHAR, SOLEIMANJAHI, HOORIEH, TAVASSOTI KHEIRI, MASOUMEH, JAMALI, ABBAS, & JAMAATI, AZAM. (2013). DETERMINING INFLUENZA VIRUS SHEDDING AT DIFFERENT TIME POINTS IN MADIN-DARBY CANINE KIDNEY CELL LINE. CELL JOURNAL (YAKHTEH), 15(2 (58)), 130-135. SID. https://sid.ir/paper/598791/en

    Vancouver: Copy

    ABDOLI ASGHAR, SOLEIMANJAHI HOORIEH, TAVASSOTI KHEIRI MASOUMEH, JAMALI ABBAS, JAMAATI AZAM. DETERMINING INFLUENZA VIRUS SHEDDING AT DIFFERENT TIME POINTS IN MADIN-DARBY CANINE KIDNEY CELL LINE. CELL JOURNAL (YAKHTEH)[Internet]. 2013;15(2 (58)):130-135. Available from: https://sid.ir/paper/598791/en

    IEEE: Copy

    ASGHAR ABDOLI, HOORIEH SOLEIMANJAHI, MASOUMEH TAVASSOTI KHEIRI, ABBAS JAMALI, and AZAM JAMAATI, “DETERMINING INFLUENZA VIRUS SHEDDING AT DIFFERENT TIME POINTS IN MADIN-DARBY CANINE KIDNEY CELL LINE,” CELL JOURNAL (YAKHTEH), vol. 15, no. 2 (58), pp. 130–135, 2013, [Online]. Available: https://sid.ir/paper/598791/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top