DESIGNING A SAFE AND NOVEL SYSTEM FOR HIV INDUCED CYTOPATHIC ASSAY USING SINGLE CYCLE REPLICABLE HIV-1 VIRIONS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ZABIHOLLAHI R.; NOURMOHAMMADI M.; FARHANG ESFAHANI A.; VAHABPOUR R.; SADAT S.M.; AGHASADEGHI M.R.; SALEHI M.; SIADAT S.D.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Ardabil University of Medical Sciences Year:2012 | Volume:12 | Issue:1 (43) Page(s): 40-50

Download Full-Text

Persian Verion

View:

10,063

Download:

Cites:

Information Journal Paper

Title

DESIGNING A SAFE AND NOVEL SYSTEM FOR HIV INDUCED CYTOPATHIC ASSAY USING SINGLE CYCLE REPLICABLE HIV-1 VIRIONS

Author(s)

Keywords

HIVQ2

SCRQ2

REPLICATION ASSAYQ2

CYTOPATHIC ASSAYQ2

Abstract

Background & Objectives: Several studies have been conducted to explore anti-HIV drugs. Discovery and study of novel anti-HIV-1 compounds need live viruses and has serious biosafety concerns. In this research we reported a novel and safe system for assaying the cytopathic effects of HIV by using single cycle replicable (SCR) HIV-1 virions.Methods: To produce the SCR HIV-1 virions, pMD2G, pmzNL4-3 and pSPAX2 plasmids were co-transfected into HEK293T cells. Different amount of SCR virions were used to infect target cells (MT-2). Within the infected cells, the number of formed syncytia was counted under the light microscopy. The lethal effects of the SCR HIV virions were measured using XTT proliferation assay.Results: Formation of syncytia among SCR HIV infected cells was detectable 24 hours after infection. Highest amount of syncytia was seen 72 hours after infection. Increase in the amount of virions caused increasing of syncytia and the cytopathic effects of SCR HIV-1. Infection with more than 1600 ng P24 SCR HIV decreased the syncytium formation and viability of all cells. The calculated IC50 (50 percent inhibitory capacity) for nevirapine and BMS806 using this method was 50 nM and 30 nM, respectively.Conclusion: SCR HIV-1 virions are replicable only for one cycle. Using these virions can improve the safety of HIV researches. Herein, we optimized the assaying of HIV induced cytopathic effects by using SCR HIV-1 (NL4-3) virions. The accuracy of this method was accepted by quantifying the anti-HIV-1 effects of nevirapine and BMS806 by (SCR) HIV-1 virions.

Cites

No record.

References

No record.

Cite

APA: Copy

ZABIHOLLAHI, R., NOURMOHAMMADI, M., FARHANG ESFAHANI, A., VAHABPOUR, R., SADAT, S.M., AGHASADEGHI, M.R., SALEHI, M., & SIADAT, S.D.. (2012). DESIGNING A SAFE AND NOVEL SYSTEM FOR HIV INDUCED CYTOPATHIC ASSAY USING SINGLE CYCLE REPLICABLE HIV-1 VIRIONS. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 12(1 (43)), 40-50. SID. https://sid.ir/paper/59909/en

Vancouver: Copy

ZABIHOLLAHI R., NOURMOHAMMADI M., FARHANG ESFAHANI A., VAHABPOUR R., SADAT S.M., AGHASADEGHI M.R., SALEHI M., SIADAT S.D.. DESIGNING A SAFE AND NOVEL SYSTEM FOR HIV INDUCED CYTOPATHIC ASSAY USING SINGLE CYCLE REPLICABLE HIV-1 VIRIONS. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2012;12(1 (43)):40-50. Available from: https://sid.ir/paper/59909/en

IEEE: Copy

R. ZABIHOLLAHI, M. NOURMOHAMMADI, A. FARHANG ESFAHANI, R. VAHABPOUR, S.M. SADAT, M.R. AGHASADEGHI, M. SALEHI, and S.D. SIADAT, “DESIGNING A SAFE AND NOVEL SYSTEM FOR HIV INDUCED CYTOPATHIC ASSAY USING SINGLE CYCLE REPLICABLE HIV-1 VIRIONS,” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 12, no. 1 (43), pp. 40–50, 2012, [Online]. Available: https://sid.ir/paper/59909/en

Related Journal Papers