THE EFFECT OF MG2+ AND MN2+ ON OVER-PRODUCTION OF INTERLEUKIN-2 IN RECOMBINANT E.COLI

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YEGANE SARKANDY SHAHIN; SHOJAOSADATI SEYED ABBAS; KHALILZADEH RASOUL; SADEGHIZADEH MAJID

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Journal Paper

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Journal: Iranian Journal of Chemistry and Chemical Engineering Year:2013 | Volume:32 | Issue:1 (65) Page(s): 127-131

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Title

THE EFFECT OF MG2+ AND MN2+ ON OVER-PRODUCTION OF INTERLEUKIN-2 IN RECOMBINANT E.COLI

Author(s)

YEGANE SARKANDY SHAHIN | SHOJAOSADATI SEYED ABBAS | KHALILZADEH RASOUL | SADEGHIZADEH MAJID | Issue Writer Certificate

Keywords

RECOMBINANT ESCHERICHIA COLI

HUMAN INTERLEUKIN-2

FULL FACTORIAL DESIGN

MAGNESIUM

MANGANESE

Abstract

In order to increase the productivity of HUMAN INTERLEUKIN-2 (IL-2) as a model protein in the recombinant E.coliBL21 (DE3), the effect of MAGNESIUM acetate and MANGANESE acetate was studied based on FULL FACTORIAL DESIGN experiments MAGNESIUM acetate and MANGANESE acetate and their interaction exhibited an increasing effect on IL-2 expression level upto 25.24%, 16.80% and 23.30%, respectively, in comparison to 10.76% for the standard M9 medium(as usual defined medium in high cell density culture). MAGNESIUM acetate was the most effective salt. Other experiments were performed to investigate the effect of MAGNESIUM and acetate ions separately. Results revealed that M9 medium with 1.5 times of MAGNESIUM ion concentration resulted in the highest expression level.

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APA: Copy

YEGANE SARKANDY, SHAHIN, SHOJAOSADATI, SEYED ABBAS, KHALILZADEH, RASOUL, & SADEGHIZADEH, MAJID. (2013). THE EFFECT OF MG²⁺ AND MN²⁺ ON OVER-PRODUCTION OF INTERLEUKIN-2 IN RECOMBINANT E.COLI. IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), 32(1 (65)), 127-131. SID. https://sid.ir/paper/601099/en

Vancouver: Copy

YEGANE SARKANDY SHAHIN, SHOJAOSADATI SEYED ABBAS, KHALILZADEH RASOUL, SADEGHIZADEH MAJID. THE EFFECT OF MG²⁺ AND MN²⁺ ON OVER-PRODUCTION OF INTERLEUKIN-2 IN RECOMBINANT E.COLI. IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE)[Internet]. 2013;32(1 (65)):127-131. Available from: https://sid.ir/paper/601099/en

IEEE: Copy

SHAHIN YEGANE SARKANDY, SEYED ABBAS SHOJAOSADATI, RASOUL KHALILZADEH, and MAJID SADEGHIZADEH, “THE EFFECT OF MG²⁺ AND MN²⁺ ON OVER-PRODUCTION OF INTERLEUKIN-2 IN RECOMBINANT E.COLI,” IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), vol. 32, no. 1 (65), pp. 127–131, 2013, [Online]. Available: https://sid.ir/paper/601099/en

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