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Information Journal Paper

Title

EVALUATING THE EXPRESSION OF SELF-RENEWAL GENES IN HUMAN ENDOTHELIAL PROGENITOR CELLS

Pages

  298-305

Abstract

 Objective: ENDOTHELIAL PROGENITOR CELLs (EPCs) have a potential application for cell therapy, however, their biological nature is not well-understood. EPCs also possess some stemness features, such as their clonogenicity and differentiation capacity. The main aim of this study was to evaluate the expression of certain transcription factors regulating self-renewal property of STEM CELLS.Materials and Methods: In this experimental study, PERIPHERAL BLOOD mononuclear cells were isolated from fresh human blood of several volunteers and were cultured in fibronectin-coated plates. EPCs were identified based on their morphology and growth characteristic. Then, the expression of some MARKERs implicated in self-renewal capacity was assessed in the isolated cells using reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry.Results: Expression of the cell surface MARKERs, CD31 and CD34, was determined by RT-PCR and immunocytochemistry. Furthermore, these cells had the ability for Di-AC-LDL incorporation as well as attachment to lectin I. EPCs did not express the main stem cell MARKERs, like OCT4-A, Nanog, and Sox2; nevertheless, they expressed the weaker pluripotent MARKERs, including OCT4B and OCT4-B1 spliced variants, such as Nucleostemin and ZFX. Furthermore, the expression of Nucleostemin and ZFX genes revealed a decreasing pattern from days 4th to 11th.Conclusion: The main regulators of stem cell self-renewal genes, including OCT4-A, Nanog, and Sox2 are not expressed in EPCs. Forced expression of these genes can elevate the stemness property and clinical application of EPCs.

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    APA: Copy

    BAYAT, HAMID, FATHI, FARDIN, PEYROVI, HABIBOLLAH, & MOWLA, SEYED JAVAD. (2013). EVALUATING THE EXPRESSION OF SELF-RENEWAL GENES IN HUMAN ENDOTHELIAL PROGENITOR CELLS. CELL JOURNAL (YAKHTEH), 14(4 (56)), 298-305. SID. https://sid.ir/paper/603486/en

    Vancouver: Copy

    BAYAT HAMID, FATHI FARDIN, PEYROVI HABIBOLLAH, MOWLA SEYED JAVAD. EVALUATING THE EXPRESSION OF SELF-RENEWAL GENES IN HUMAN ENDOTHELIAL PROGENITOR CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2013;14(4 (56)):298-305. Available from: https://sid.ir/paper/603486/en

    IEEE: Copy

    HAMID BAYAT, FARDIN FATHI, HABIBOLLAH PEYROVI, and SEYED JAVAD MOWLA, “EVALUATING THE EXPRESSION OF SELF-RENEWAL GENES IN HUMAN ENDOTHELIAL PROGENITOR CELLS,” CELL JOURNAL (YAKHTEH), vol. 14, no. 4 (56), pp. 298–305, 2013, [Online]. Available: https://sid.ir/paper/603486/en

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