FREQUENCY OF ENTEROTOXIGENIC ESCHERICHIA COLI ISOLATES HARBORING ELTB AND ELTA GENES IN DIARRHEAL SPECIMENS AMONG CHILDREN YOUNGER THAN 5 YEARS IN TABRIZ HOSPITALS

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KASHEFIYEH M.; ZEIGHAMI H.; HAGHI F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Journal of Advances in Medical and Biomedical Research Year:2014 | Volume:21 | Issue:88 Page(s): 120-127

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Title

FREQUENCY OF ENTEROTOXIGENIC ESCHERICHIA COLI ISOLATES HARBORING ELTB AND ELTA GENES IN DIARRHEAL SPECIMENS AMONG CHILDREN YOUNGER THAN 5 YEARS IN TABRIZ HOSPITALS

Author(s)

KASHEFIYEH M. | ZEIGHAMI H. | HAGHI F. | Issue Writer Certificate

Keywords

DIARRHEAQ2

ENTEROTOXIGENIC ESCHERICHIA COLIQ2

ELTBQ2

ESTAQ2

PCRQ2

Abstract

Background and Objective: ENTEROTOXIGENIC ESCHERICHIA COLI (ETEC) have been recognized as the most common cause of infectious DIARRHEA in infants and children less than five years in developing countries. The aim of the present study was to determine the frequency of ETEC isolates harboring ELTB and ESTA genes among DIARRHEAl children admitted to hospitals in Tabriz, Iran.Materials and Methods: In this cross sectional study, during the 7 month, a total of 450 stool samples of children younger than 5 years of age were studied. After culture and confirming, the DNA of Escherichia coli isolates were extracted by boiling method and multiplex PCR was done for detection of ESTA, ELTB genes.Results: A total of 200 DIARRHEAgenic E. coli strains were isolated from 450 stool samples from children with DIARRHEA. The prevalence of DIARRHEAgenic E. coli isolated from stool samples was 44.44%. In order to detect ELTB, ESTA genes simultaneously, a mixture of two primer pairs specific for the target genes was used in a single PCR. In our study, PCR assays detected 75 (37.5%) ETEC isolates. Of the ETEC strains isolated, 43 (21.5%) isolates produced heat labile toxin only, 11 (5.5%) produced heat-labile toxin and heat-stable toxin, and 21 (10.5%) produced heat stable toxin only.Conclusion: Due to the frequency distribution of enterotoxigenic isolates in Tabriz, particularly in children younger than 5 years of age, the use of molecular methods in diagnostic laboratories is recommended.

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APA: Copy

KASHEFIYEH, M., ZEIGHAMI, H., & HAGHI, F.. (2014). FREQUENCY OF ENTEROTOXIGENIC ESCHERICHIA COLI ISOLATES HARBORING ELTB AND ELTA GENES IN DIARRHEAL SPECIMENS AMONG CHILDREN YOUNGER THAN 5 YEARS IN TABRIZ HOSPITALS. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, 21(88), 120-127. SID. https://sid.ir/paper/61617/en

Vancouver: Copy

KASHEFIYEH M., ZEIGHAMI H., HAGHI F.. FREQUENCY OF ENTEROTOXIGENIC ESCHERICHIA COLI ISOLATES HARBORING ELTB AND ELTA GENES IN DIARRHEAL SPECIMENS AMONG CHILDREN YOUNGER THAN 5 YEARS IN TABRIZ HOSPITALS. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH[Internet]. 2014;21(88):120-127. Available from: https://sid.ir/paper/61617/en

IEEE: Copy

M. KASHEFIYEH, H. ZEIGHAMI, and F. HAGHI, “FREQUENCY OF ENTEROTOXIGENIC ESCHERICHIA COLI ISOLATES HARBORING ELTB AND ELTA GENES IN DIARRHEAL SPECIMENS AMONG CHILDREN YOUNGER THAN 5 YEARS IN TABRIZ HOSPITALS,” JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, vol. 21, no. 88, pp. 120–127, 2014, [Online]. Available: https://sid.ir/paper/61617/en

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