MIR-451 UP-REGULATION, INDUCE ERYTHROID DIFFERENTIATION OF CD133+CELLS INDEPENDENT OF CYTOKINE COCKTAILS

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KOUHKAN FATEMEH; SOLEIMANI MASOUD; DALIRI MORTEZA; BEHMANESH MEHRDAD; MOBARRA NASER; MOSSAHEBI MOHAMMADI MAJID; MOHAMMADI SHAHIN; MOKHTARI MEHDI; LAHMY REYHANEH

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Journal: Iranian Journal of Basic Medical Sciences Year:2013 | Volume:16 | Issue:6 Page(s): 756-762

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Title

MIR-451 UP-REGULATION, INDUCE ERYTHROID DIFFERENTIATION OF CD133+CELLS INDEPENDENT OF CYTOKINE COCKTAILS

Author(s)

Keywords

ERYTHROPOIESIS

CD133++

MICRORNA

MIR-451

MIR-16

Abstract

Objective(s): ERYTHROPOIESIS is regulated by some extrinsic and intrinsic factors as MICRORNAs (miRNAs). miRNAs are endogenously small non-coding regulatory RNAs which play vital roles in the variety of cellular fate, critical processes; growth, apoptosis, metabolism, survival of the cells and specially differentiation. Several miRNAs such as MIR-16 and MIR-451 have been shown to be correlated with erythroid differentiation. Taking into account the importance of miRNAs in cellular differentiation, the goal of the present study was to examine the role of miRNAs in hematopoietic stem cells (HSC) differentiation into the erythroid cells in the absence of growth factors and stimulatory cytokines.Materials and Methods: CD133++ stem cells were infected with lentiviruses containing MIR-451/miR-16 precursor sequence, erythroid differentiation was evaluated using RT-PCR for hemoglobin chains and surface antigens, also by banzidine staining.Results: MIR-451up-regulation, but not MIR-16, could induce a, b and g-globin expression in CD133++ cells and have strong correlation with appearance of CD71 and CD235a markers in these cells. Moreover, MIR-451 up-regulation increases the banzidine positive cells to ~ %40.Conclusion: Our results provide strong evidence that MIR-451 up-regulation strongly induces erythroid differentiation and maturation of CD133++ stem cells. Hence, this method may provide a useful technique for the production of artificial blood RBC and be used as a new strategy for gene therapy of hemoglobinopathies, such as b-thalassemias and sickle cell anemia.

Cites

Simultaneous regulation of miR-451 and miR-191 led to erythroid fate decision of mouse embryonic stem cell

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APA: Copy

KOUHKAN, FATEMEH, SOLEIMANI, MASOUD, DALIRI, MORTEZA, BEHMANESH, MEHRDAD, MOBARRA, NASER, MOSSAHEBI MOHAMMADI, MAJID, MOHAMMADI, SHAHIN, MOKHTARI, MEHDI, & LAHMY, REYHANEH. (2013). MIR-451 UP-REGULATION, INDUCE ERYTHROID DIFFERENTIATION OF CD133+CELLS INDEPENDENT OF CYTOKINE COCKTAILS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 16(6), 756-762. SID. https://sid.ir/paper/629409/en

Vancouver: Copy

KOUHKAN FATEMEH, SOLEIMANI MASOUD, DALIRI MORTEZA, BEHMANESH MEHRDAD, MOBARRA NASER, MOSSAHEBI MOHAMMADI MAJID, MOHAMMADI SHAHIN, MOKHTARI MEHDI, LAHMY REYHANEH. MIR-451 UP-REGULATION, INDUCE ERYTHROID DIFFERENTIATION OF CD133+CELLS INDEPENDENT OF CYTOKINE COCKTAILS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2013;16(6):756-762. Available from: https://sid.ir/paper/629409/en

IEEE: Copy

FATEMEH KOUHKAN, MASOUD SOLEIMANI, MORTEZA DALIRI, MEHRDAD BEHMANESH, NASER MOBARRA, MAJID MOSSAHEBI MOHAMMADI, SHAHIN MOHAMMADI, MEHDI MOKHTARI, and REYHANEH LAHMY, “MIR-451 UP-REGULATION, INDUCE ERYTHROID DIFFERENTIATION OF CD133+CELLS INDEPENDENT OF CYTOKINE COCKTAILS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 16, no. 6, pp. 756–762, 2013, [Online]. Available: https://sid.ir/paper/629409/en

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