SDF-1α/CXCR4 AXIS MEDIATES THE MIGRATION OF MESENCHYMAL STEM CELLS TO THE HYPOXIC-ISCHEMIC BRAIN LESION IN A RAT MODEL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

YU QIN; LIU LIZHEN; LIN JIE; WANG YAN; XUAN XIAOBO; GUO YING; HU SHAOJUN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2015 | Volume:16 | Issue:4 (64) Page(s): 440-447

Download Full-Text

View:

519

Download:

293

Cites:

Information Journal Paper

Title

SDF-1α/CXCR4 AXIS MEDIATES THE MIGRATION OF MESENCHYMAL STEM CELLS TO THE HYPOXIC-ISCHEMIC BRAIN LESION IN A RAT MODEL

Author(s)

Keywords

MESENCHYMAL STEM CELLS

MIGRATION

SDF-1α

CXCR 4

Abstract

Objective: Transplantation of MESENCHYMAL STEM CELLS (MSCs) can promote functional recovery of the brain after hypoxic-ischemic brain damage (HIBD). However, the mechanism regulating MSC MIGRATION to a hypoxic-ischemic lesion is poorly understood. Interaction between stromal cell-derived factor-1a (SDF-1a) and its cognate receptor CXC chemokine receptor 4 (CXCR4) is crucial for homing and MIGRATION of multiple stem cell types. In this study, we investigate the potential role of SDF-1a/CXCR4 axis in mediating MSC MIGRATION in an HIBD model.Materials and Methods: In this experimental study, we first established the animal model of HIBD using the neonatal rat. Bone marrow MSCs were cultured and labeled with 5-bromo-21-deoxyuridine (BrdU) after which 6×106 cells were intravenously injected into the rat. BrdU positive MSCs in the hippocampus were detected by immunohistochemical analyses. The expression of hypoxia-inducible factor-1a (HIF-1a) and SDF-1a in the hippocampus of hypoxic-ischemic rats was detected by Western blotting. To investigate the role of hypoxia and SDF-1a on MIGRATION of MSCs in vitro, MSCs isolated from normal rats were cultured in a hypoxic environment (PO2=1%). MIGRATION of MSCs was detected by the transwell assay. The expression of CXCR4 was tested using Western blotting and flow cytometry.Results: BrdU-labeled MSCs were found in the rat brain, which suggested that transplanted MSCs migrated to the site of the hypoxic-ischemic brain tissue. HIF-1a and SDF- 1a significantly increased in the hippocampal formations of HIBD rats in a time-dependent manner. They peaked on day 7 and were stably expressed until day 21. MIGRATION of MSCs in vitro was promoted by SDF-1a under hypoxia and inhibited by the CXCR4 inhibitor AMD3100. The expression of CXCR4 on MSCs was elevated by hypoxia stimulation as well as microdosage treatment of SDF-1a.Conclusion: This observation illustrates that SDF-1a/CXCR4 axis mediate the MIGRATION of MSCs to a hypoxic-ischemic brain lesion in a rat model.

Cites

No record.

References

Cite

APA: Copy

YU, QIN, LIU, LIZHEN, LIN, JIE, WANG, YAN, XUAN, XIAOBO, GUO, YING, & HU, SHAOJUN. (2015). SDF-1α/CXCR4 AXIS MEDIATES THE MIGRATION OF MESENCHYMAL STEM CELLS TO THE HYPOXIC-ISCHEMIC BRAIN LESION IN A RAT MODEL. CELL JOURNAL (YAKHTEH), 16(4 (64)), 440-447. SID. https://sid.ir/paper/630172/en

Vancouver: Copy

YU QIN, LIU LIZHEN, LIN JIE, WANG YAN, XUAN XIAOBO, GUO YING, HU SHAOJUN. SDF-1α/CXCR4 AXIS MEDIATES THE MIGRATION OF MESENCHYMAL STEM CELLS TO THE HYPOXIC-ISCHEMIC BRAIN LESION IN A RAT MODEL. CELL JOURNAL (YAKHTEH)[Internet]. 2015;16(4 (64)):440-447. Available from: https://sid.ir/paper/630172/en

IEEE: Copy

QIN YU, LIZHEN LIU, JIE LIN, YAN WANG, XIAOBO XUAN, YING GUO, and SHAOJUN HU, “SDF-1α/CXCR4 AXIS MEDIATES THE MIGRATION OF MESENCHYMAL STEM CELLS TO THE HYPOXIC-ISCHEMIC BRAIN LESION IN A RAT MODEL,” CELL JOURNAL (YAKHTEH), vol. 16, no. 4 (64), pp. 440–447, 2015, [Online]. Available: https://sid.ir/paper/630172/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops