EFFECTS OF LIPOPOLYSACCHARIDES FROM DIFFERENT SHIGELLA STRAINS ON TOLL-LIKE RECEPTOR 4 EXPRESSION IN HUMAN BONE MARROW MESENCHYMAL STEM CELLS; POSTER PRESENTATION

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HASHEMZADEH M.R.; AFLATOONIAN R.; SAADATI M.; BAGHBAN ESLAMINEZHAD M.R.; ZAREA M.

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Journal: Cell Journal (Yakhteh) Year:2012 | Volume:14 | Issue:SUPPLEMENT 1 Page(s): 45-45

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Title

EFFECTS OF LIPOPOLYSACCHARIDES FROM DIFFERENT SHIGELLA STRAINS ON TOLL-LIKE RECEPTOR 4 EXPRESSION IN HUMAN BONE MARROW MESENCHYMAL STEM CELLS; POSTER PRESENTATION

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Keywords

HUMAN BONE MARROW DERIVED MESENCHYMAL STEM CELLS

TLR4

LPS

SHIGELLA

Abstract

Objective: Toll-like receptors (TLRs) are a conserved family of receptors that recognize pathogen-associated molecular patterns and promote the activation of immune cells. To date, several TLRs (numbered 1–11) have been identified in humans. One such receptor– ligand complex is formed between the mammalian Toll-like receptor 4 (TLR4) –MD2–CD14 complex and bacterial lipopolysaccharide (LPS). Initially, research on TLRs focused on their expression and signaling consequences in immune cells. However, recent reports indicate that other bone marrow-derived cells, including mesenchymal stem/progenitor cells (MSCs), are among the cells that express TLR proteins. MSCs are separated from other cells in the bone marrow by their tendency to adhere to plastic.Materials and Methods: In this study, the effects of LPS from different SHIGELLA strains (S. flexnery, S. dysentriae and S. sonnei) with various concentration include of 0.1, 1 and 10 mg/ml on TLR4 expression in human bone marrow derived mesenchymal stem cell was evaluated by RT-PCR and Q-PCR.Results: RT-PCR has not shown TLR4 expression in control group in contrast of all case group. Q-PCR data has shown all of lipopolysaccharides from SHIGELLA strains with varies concentration led to TLR4 expression. However, the highest expression was shown in 10mg/ml of S.flexneri treatment and the lowest one in 0.1 mg/ml SHIGELLA sonnei treatment.Conclusion: The innate immune response induced by Gram negative bacterial LPS as a TLR4 agonist. LPS composition varies widely among different species of Gram negative bacteria. Therefore, it expected that LPS from different SHIGELLA strains is leading to different expression levels of TLR4. Our data confirm this expectation. In addition, Q-PCR data was shown ascending levels of TLR4 expression with increasing of LPS concentration from all of SHIGELLA strains.

Cites

MULTIPLE GENOTYPES OF THE COMMONLY CO-SEGREGATING TOLL-LIKE RECEPTOR 4 ASP299GLY AND THR399ILE IN BALUCHI MALARIA PATIENTS FROM IRAN

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APA: Copy

HASHEMZADEH, M.R., AFLATOONIAN, R., SAADATI, M., BAGHBAN ESLAMINEZHAD, M.R., & ZAREA, M.. (2012). EFFECTS OF LIPOPOLYSACCHARIDES FROM DIFFERENT SHIGELLA STRAINS ON TOLL-LIKE RECEPTOR 4 EXPRESSION IN HUMAN BONE MARROW MESENCHYMAL STEM CELLS; POSTER PRESENTATION. CELL JOURNAL (YAKHTEH), 14(SUPPLEMENT 1), 45-45. SID. https://sid.ir/paper/630467/en

Vancouver: Copy

HASHEMZADEH M.R., AFLATOONIAN R., SAADATI M., BAGHBAN ESLAMINEZHAD M.R., ZAREA M.. EFFECTS OF LIPOPOLYSACCHARIDES FROM DIFFERENT SHIGELLA STRAINS ON TOLL-LIKE RECEPTOR 4 EXPRESSION IN HUMAN BONE MARROW MESENCHYMAL STEM CELLS; POSTER PRESENTATION. CELL JOURNAL (YAKHTEH)[Internet]. 2012;14(SUPPLEMENT 1):45-45. Available from: https://sid.ir/paper/630467/en

IEEE: Copy

M.R. HASHEMZADEH, R. AFLATOONIAN, M. SAADATI, M.R. BAGHBAN ESLAMINEZHAD, and M. ZAREA, “EFFECTS OF LIPOPOLYSACCHARIDES FROM DIFFERENT SHIGELLA STRAINS ON TOLL-LIKE RECEPTOR 4 EXPRESSION IN HUMAN BONE MARROW MESENCHYMAL STEM CELLS; POSTER PRESENTATION,” CELL JOURNAL (YAKHTEH), vol. 14, no. SUPPLEMENT 1, pp. 45–45, 2012, [Online]. Available: https://sid.ir/paper/630467/en

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