COMPARISON BETWEEN CHONDROGENIC MARKERS OF DIFFERENTIATED CHONDROCYTES FROM ADIPOSE DERIVED STEM CELLS AND ARTICULAR CHONDROCYTES IN VITRO

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MARDANI MOHMMAD; HASHEMIBENI BATOOL; ANSAR MALEK MASOUD; ZARKESH ESFAHANI SAYEED HAMID; KAZEMI MOHAMMAD; GOHARIAN VAHID; ESMAEILI NAFISEH; ESFANDIARY EBRAHIM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2013 | Volume:16 | Issue:6 Page(s): 763-771

Download Full-Text

View:

313

Download:

248

Cites:

Information Journal Paper

Title

COMPARISON BETWEEN CHONDROGENIC MARKERS OF DIFFERENTIATED CHONDROCYTES FROM ADIPOSE DERIVED STEM CELLS AND ARTICULAR CHONDROCYTES IN VITRO

Author(s)

Keywords

ALGINATE

STEM CELL

CHONDROCYTE

TGFBETA

Abstract

Objective(s): Osteoarthritis is one of the most common diseases in middle-aged population in the world. Cartilage tissue engineering (TE) has been presented as an effort to introduce the best combination of cells, biomaterial scaffolds and stimulating growth factors to produce a cartilage tissue similar to the natural articular cartilage. In this study, the chondrogenic potent ial of adipose derived STEM CELLs (ADSCs) was compared with natural articular CHONDROCYTEs cultured in ALGINATE scaffold.Materials and Methods: Human ADSCs were obtained from subcutaneous adipose tissue and human articular CHONDROCYTEs from non-weight bearing areas of knee joints. Cells were seeded in 1.5% ALGINATE and cultured in chondrogenic media for three weeks with and without TGFb3. The genes expression of types II and X collagens was assessed by Real Time PCR and the amount of aggrecan (AGC) and type I collagen measured by ELISA and the content of glycosaminoglycan evaluated by GAG assay.Results: Our findings showed that type II collagen, GAG and AGC were expressed, in differentiated ADSCs. Meanwhile, they produced a lesser amount of types II and X collagens but more AGC, GAG and type I collagen in comparison with natural CHONDROCYTEs (NCs).Conclusion: Further attempt should be carried out to optimize achieving type II collagen in DCs, as much as, natural articular CHONDROCYTEs and decline of the production of type I collagen in order to provide efficient hyaline cartilage after chondrogenic induction, prior to the usage of harvested tissues in clinical trials.

Cites

No record.

References

No record.

Cite

APA: Copy

MARDANI, MOHMMAD, HASHEMIBENI, BATOOL, ANSAR, MALEK MASOUD, ZARKESH ESFAHANI, SAYEED HAMID, KAZEMI, MOHAMMAD, GOHARIAN, VAHID, ESMAEILI, NAFISEH, & ESFANDIARY, EBRAHIM. (2013). COMPARISON BETWEEN CHONDROGENIC MARKERS OF DIFFERENTIATED CHONDROCYTES FROM ADIPOSE DERIVED STEM CELLS AND ARTICULAR CHONDROCYTES IN VITRO. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 16(6), 763-771. SID. https://sid.ir/paper/630564/en

Vancouver: Copy

MARDANI MOHMMAD, HASHEMIBENI BATOOL, ANSAR MALEK MASOUD, ZARKESH ESFAHANI SAYEED HAMID, KAZEMI MOHAMMAD, GOHARIAN VAHID, ESMAEILI NAFISEH, ESFANDIARY EBRAHIM. COMPARISON BETWEEN CHONDROGENIC MARKERS OF DIFFERENTIATED CHONDROCYTES FROM ADIPOSE DERIVED STEM CELLS AND ARTICULAR CHONDROCYTES IN VITRO. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2013;16(6):763-771. Available from: https://sid.ir/paper/630564/en

IEEE: Copy

MOHMMAD MARDANI, BATOOL HASHEMIBENI, MALEK MASOUD ANSAR, SAYEED HAMID ZARKESH ESFAHANI, MOHAMMAD KAZEMI, VAHID GOHARIAN, NAFISEH ESMAEILI, and EBRAHIM ESFANDIARY, “COMPARISON BETWEEN CHONDROGENIC MARKERS OF DIFFERENTIATED CHONDROCYTES FROM ADIPOSE DERIVED STEM CELLS AND ARTICULAR CHONDROCYTES IN VITRO,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 16, no. 6, pp. 763–771, 2013, [Online]. Available: https://sid.ir/paper/630564/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops