BRIEF AZACYTIDINE STEP ALLOWS THE CONVERSION OF SUSPENSION HUMAN FIBROBLASTS INTO NEURAL PROGENITOR-LIKE CELLS

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MIRAKHORI FAHIMEH; ZEYNALI BAHMAN; KIANI SAHAR; BAHARVAND HOSSEIN

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Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2015 | Volume:17 | Issue:1 (65) Page(s): 153-158

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Title

BRIEF AZACYTIDINE STEP ALLOWS THE CONVERSION OF SUSPENSION HUMAN FIBROBLASTS INTO NEURAL PROGENITOR-LIKE CELLS

Author(s)

MIRAKHORI FAHIMEH | ZEYNALI BAHMAN | KIANI SAHAR | BAHARVAND HOSSEIN | Issue Writer Certificate

Keywords

AZACYTIDINE

FIBROBLAST

PROGENITOR CELLS

TRANSDIFFERENTIATION

Abstract

In recent years TRANSDIFFERENTIATION technology has enabled direct conversion of human FIBROBLASTs to become a valuable, abundant and accessible cell source for patient-specific induced cell generation in biomedical research. The majority of TRANSDIFFERENTIATION approaches rely upon viral gene delivery which due to random integration with the host genome can cause genome instability and tumorigenesis upon transplantation. Here, we provide a simple way to induce neural progenitor-like cells from human FIBROBLASTs without genetic manipulation by changing physicochemical culture properties from monolayer culture into a suspension in the presence of a chemical DNA methyltransferase inhibitor agent, AZACYTIDINE. We have demonstrated the expression of neural progenitor-like markers, morphology and the ability to spontaneously differentiate into neural-like cells. This approach is simple, inexpensive, lacks genetic manipulation and could be a foundation for future chemical neural TRANSDIFFERENTIATION and a safe induction of neural PROGENITOR CELLS from human FIBROBLASTs for clinical applications.

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APA: Copy

MIRAKHORI, FAHIMEH, ZEYNALI, BAHMAN, KIANI, SAHAR, & BAHARVAND, HOSSEIN. (2015). BRIEF AZACYTIDINE STEP ALLOWS THE CONVERSION OF SUSPENSION HUMAN FIBROBLASTS INTO NEURAL PROGENITOR-LIKE CELLS. CELL JOURNAL (YAKHTEH), 17(1 (65)), 153-158. SID. https://sid.ir/paper/633497/en

Vancouver: Copy

MIRAKHORI FAHIMEH, ZEYNALI BAHMAN, KIANI SAHAR, BAHARVAND HOSSEIN. BRIEF AZACYTIDINE STEP ALLOWS THE CONVERSION OF SUSPENSION HUMAN FIBROBLASTS INTO NEURAL PROGENITOR-LIKE CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2015;17(1 (65)):153-158. Available from: https://sid.ir/paper/633497/en

IEEE: Copy

FAHIMEH MIRAKHORI, BAHMAN ZEYNALI, SAHAR KIANI, and HOSSEIN BAHARVAND, “BRIEF AZACYTIDINE STEP ALLOWS THE CONVERSION OF SUSPENSION HUMAN FIBROBLASTS INTO NEURAL PROGENITOR-LIKE CELLS,” CELL JOURNAL (YAKHTEH), vol. 17, no. 1 (65), pp. 153–158, 2015, [Online]. Available: https://sid.ir/paper/633497/en

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