LIPOPOLYSACCHARIDE- AND LIPOTEICHOIC ACID-MEDIATED PRO-INFLAMMATORY CYTOKINE PRODUCTION AND MODULATION OF TLR2, TLR4 AND MYD88 EXPRESSION IN HUMAN ENDOMETRIAL CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

RASHIDI NESA; MIRAHMADIAN MAHROO; JEDDI TEHRANI MAHMOOD; REZANIA SIMIN; GHASEMI JAMILEH; KAZEMNEJAD SOMAIEH; MIRZADEGAN EBRAHIM; VAFAEI SEDIGHEH; KASHANIAN MARYAM; RASOULZADEH ZAHRA; ZARNANI AMIR HASSAN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Reproduction & Infertility Year:2015 | Volume:16 | Issue:2 Page(s): 72-81

Download Full-Text

View:

395

Download:

143

Cites:

Information Journal Paper

Title

LIPOPOLYSACCHARIDE- AND LIPOTEICHOIC ACID-MEDIATED PRO-INFLAMMATORY CYTOKINE PRODUCTION AND MODULATION OF TLR2, TLR4 AND MYD88 EXPRESSION IN HUMAN ENDOMETRIAL CELLS

Author(s)

Keywords

CYTOKINE

ENDOMETRIUM

INFLAMMATION

LPS

LTA

TOLL-LIKE RECEPTOR

Abstract

Background: TOLL-LIKE RECEPTOR (TLR)-mediated inflammatory processes are supposed to be involved in pathophysiology of spontaneous abortion and preterm labor. Here, we investigated functional responses of human endometrial stromal cells (ESCs) and whole endometrial cells (WECs) to lipopolysaccharide (LPS) and lipoteichoic acid (LTA).Methods: Endometrial tissues were obtained from 15 cycling women who underwent laparoscopic tubal ligation. Modulation of TLR2, TLR4 and MyD88 expression and production of pro-inflammatory CYTOKINEs by WECs and ESCs in response to LPS and LTA were assessed.Results: WECs and ESCs expressed significant levels of TLR4 and MyD88 transcripts but, unlike WECs, ESCs failed to express TLR2 gene. Regardless of positive results of Western blotting, ESCs did not express TLR4 at their surface as judged by flow cytome-try. Immunofluorescent staining revealed intracellular localization of TLR4 with pre-dominant perinuclear pattern. LPS stimulation marginally increased TLR4 gene expression in both cell types, whereas such treatment significantly upregulated MyD88 gene expression after 8 hr (p<0.05). At the protein level, however, LPS activation significantly increased TLR4 expression by ESCs (p<0.05). LTA stimulation of WECs was accompanied with non-significant increase of TLR2 and MyD88 transcripts. LPS and LTA stimulation of WECs caused significant production of IL-6 and IL-8 in a dose-dependent manner (p<0.05). Similarly, ESCs produced significant amounts of IL-6, IL-8 and also TNF-α in response to LPS activation (p<0.05).Conclusion: Our results provided further evidence of initiation of inflammatory processes following endometrial TLR activation by bacterial components which could potentially be harmful to developing fetus.

Cites

No record.

References

Cite

APA: Copy

RASHIDI, NESA, MIRAHMADIAN, MAHROO, JEDDI TEHRANI, MAHMOOD, REZANIA, SIMIN, GHASEMI, JAMILEH, KAZEMNEJAD, SOMAIEH, MIRZADEGAN, EBRAHIM, VAFAEI, SEDIGHEH, KASHANIAN, MARYAM, RASOULZADEH, ZAHRA, & ZARNANI, AMIR HASSAN. (2015). LIPOPOLYSACCHARIDE- AND LIPOTEICHOIC ACID-MEDIATED PRO-INFLAMMATORY CYTOKINE PRODUCTION AND MODULATION OF TLR2, TLR4 AND MYD88 EXPRESSION IN HUMAN ENDOMETRIAL CELLS. JOURNAL OF REPRODUCTION AND INFERTILITY, 16(2), 72-81. SID. https://sid.ir/paper/636973/en

Vancouver: Copy

RASHIDI NESA, MIRAHMADIAN MAHROO, JEDDI TEHRANI MAHMOOD, REZANIA SIMIN, GHASEMI JAMILEH, KAZEMNEJAD SOMAIEH, MIRZADEGAN EBRAHIM, VAFAEI SEDIGHEH, KASHANIAN MARYAM, RASOULZADEH ZAHRA, ZARNANI AMIR HASSAN. LIPOPOLYSACCHARIDE- AND LIPOTEICHOIC ACID-MEDIATED PRO-INFLAMMATORY CYTOKINE PRODUCTION AND MODULATION OF TLR2, TLR4 AND MYD88 EXPRESSION IN HUMAN ENDOMETRIAL CELLS. JOURNAL OF REPRODUCTION AND INFERTILITY[Internet]. 2015;16(2):72-81. Available from: https://sid.ir/paper/636973/en

IEEE: Copy

NESA RASHIDI, MAHROO MIRAHMADIAN, MAHMOOD JEDDI TEHRANI, SIMIN REZANIA, JAMILEH GHASEMI, SOMAIEH KAZEMNEJAD, EBRAHIM MIRZADEGAN, SEDIGHEH VAFAEI, MARYAM KASHANIAN, ZAHRA RASOULZADEH, and AMIR HASSAN ZARNANI, “LIPOPOLYSACCHARIDE- AND LIPOTEICHOIC ACID-MEDIATED PRO-INFLAMMATORY CYTOKINE PRODUCTION AND MODULATION OF TLR2, TLR4 AND MYD88 EXPRESSION IN HUMAN ENDOMETRIAL CELLS,” JOURNAL OF REPRODUCTION AND INFERTILITY, vol. 16, no. 2, pp. 72–81, 2015, [Online]. Available: https://sid.ir/paper/636973/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops