MICRORNAS THAT TARGET RGS5

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BANAEI ESFAHANI AMIR; MOAZZENI HAMIDREZA; NASERI NOSAR POOYA; AMIN SADAF; AREFIAN EHSAN; SOLEIMANI MASOUD; YAZDANI SHAHIN; ELAHI ELAHE

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Journal: Iranian Journal of Basic Medical Sciences Year:2015 | Volume:18 | Issue:2 Page(s): 108-114

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Title

MICRORNAS THAT TARGET RGS5

Author(s)

Keywords

MIR-7

MIR-9

MIR-23A

MIR-23B

MIR-96

MIR-204

MIR-211

RGS5

Abstract

Objective(s): An earlier meta-analysis on gene expression data derived from four microarray, two cDNA library, and one SAGE experiment had identified RGS5 as one of only ten non-housekeeping genes that were reported to be expressed in human trabecular meshwork (TM) cells by all studies. RGS5 encodes regulator of G-protein signaling-5. The TM tissue is the route of aqueous fluid outflow, and is relevant to the pathology of glaucoma. MicroRNAs constitute the most recently identified components of the cellular machinery for gene regulation in eukaryotic cells. Given our long standing interest in glaucoma, we aimed to identify miRNAs that may target RGS5.Materials and Methods: Eight miRNAs were selected for study using bioinformatics tools and available data on miRNAs expressed in the eye. Their effects were assessed using the dual luciferase assay. 3’-UTR segments of RGS5 mRNA were cloned downstream of a luciferase coding gene in psiCHECK2 vectors, and these were co-transfected with each of the miRNAs into HEK293 cells.Results: The outcomes evidenced that one or more of the segments are in fact targeted by MIR-7, MIR-9, MIR-96, MIR-23A, MIR-23B, MIR-204, and MIR-211. Down regulations by the miRNAs were statistically significant. The effect of MIR-204 is considered particularly important as this miRNA is well known to regulate eye development and to affect multiple ocular functions.Conclusion: Our results justify further studies on regulation of RGS5 expression and RGS5 downstream functions by these miRNAs.

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APA: Copy

BANAEI ESFAHANI, AMIR, MOAZZENI, HAMIDREZA, NASERI NOSAR, POOYA, AMIN, SADAF, AREFIAN, EHSAN, SOLEIMANI, MASOUD, YAZDANI, SHAHIN, & ELAHI, ELAHE. (2015). MICRORNAS THAT TARGET RGS5. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 18(2), 108-114. SID. https://sid.ir/paper/638263/en

Vancouver: Copy

BANAEI ESFAHANI AMIR, MOAZZENI HAMIDREZA, NASERI NOSAR POOYA, AMIN SADAF, AREFIAN EHSAN, SOLEIMANI MASOUD, YAZDANI SHAHIN, ELAHI ELAHE. MICRORNAS THAT TARGET RGS5. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2015;18(2):108-114. Available from: https://sid.ir/paper/638263/en

IEEE: Copy

AMIR BANAEI ESFAHANI, HAMIDREZA MOAZZENI, POOYA NASERI NOSAR, SADAF AMIN, EHSAN AREFIAN, MASOUD SOLEIMANI, SHAHIN YAZDANI, and ELAHE ELAHI, “MICRORNAS THAT TARGET RGS5,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 18, no. 2, pp. 108–114, 2015, [Online]. Available: https://sid.ir/paper/638263/en

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