QUANTIFICATION OF OXYTOCIN IN PHARMACEUTICAL DOSAGE FORMS AVAILABLE IN DRUG MARKET OF IRAN BY USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD (HPLC)

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AKBARI AZAM; HEYDARI AMIR

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Journal: Studies in Medical Sciences Year:2014 | Volume:24 | Issue:12 Page(s): 956-965

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Title

QUANTIFICATION OF OXYTOCIN IN PHARMACEUTICAL DOSAGE FORMS AVAILABLE IN DRUG MARKET OF IRAN BY USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD (HPLC)

Author(s)

AKBARI AZAM | HEYDARI AMIR | Issue Writer Certificate

Keywords

OXYTOCINQ3

HPLCQ2

PHARMACEUTICAL DOSAGE FORMQ2

ULTRA VIOLET DETECTORQ2

Abstract

Background & Aims: Quality control of medicinal products- especially the amount of active ingredients of toxic side effects and complications- is important. The present study was performed to measure OXYTOCIN (OT) in commercially available drug products in the drug market of Iran.Materials & Methods: In this study PHARMACEUTICAL DOSAGE FORM of OT with different brands at a concentration of 5 and 10 IU/ml was prepared from a local pharmacy. A previously published method was optimized for the conditions in our laboratory. High Performance Liquid Chromatography (HPLC) was used as the preferred analytical tool. The method used a C18 column. The mobile phase consist of acetonitrile/ phosphate buffer with (pH=5, 0.08 M) (20:80) and UV detection at 220 nm.Results: The results of these experiments showed a linear standard curve for OT over the range of 0.5-12 IU/ml. The corresponding regression equation was A=133.1C-2.193 with an r2 value of 0.99. The Inter-day coefficient of variation for the method ranged between 1.97 IU/ml and 9.93 IU/ml% and the Intra-day value ranged between 1.10 IU/ml and 1.76 IU/ml. The accuracy of method range was between 96.40% and 107.5% for Inter-day analysis and 93% and 99.6 % for Intra-days analysis. The lower limit of quantification was 0.25 IU/ml. The percentage content, taking one of the bulk samples as 100% references, was 107-120% and 114.2-216.6% recovery of the label claim for OT of 10IU/ml and OT of 5 IU/ml, respectively.Conclusion: These findings confirm that the amount of OXYTOCIN in some of dosage forms is higher than the declared amounts. This study emphasizes on the necessity of quality and accurate control s over the industrial pharmaceutical.

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APA: Copy

AKBARI, AZAM, & HEYDARI, AMIR. (2014). QUANTIFICATION OF OXYTOCIN IN PHARMACEUTICAL DOSAGE FORMS AVAILABLE IN DRUG MARKET OF IRAN BY USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD (HPLC). STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), 24(12), 956-965. SID. https://sid.ir/paper/63894/en

Vancouver: Copy

AKBARI AZAM, HEYDARI AMIR. QUANTIFICATION OF OXYTOCIN IN PHARMACEUTICAL DOSAGE FORMS AVAILABLE IN DRUG MARKET OF IRAN BY USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD (HPLC). STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES)[Internet]. 2014;24(12):956-965. Available from: https://sid.ir/paper/63894/en

IEEE: Copy

AZAM AKBARI, and AMIR HEYDARI, “QUANTIFICATION OF OXYTOCIN IN PHARMACEUTICAL DOSAGE FORMS AVAILABLE IN DRUG MARKET OF IRAN BY USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD (HPLC),” STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), vol. 24, no. 12, pp. 956–965, 2014, [Online]. Available: https://sid.ir/paper/63894/en

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