PREVALENCE OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS BY DISK DIFFUSION AND MOLECULAR METHODS IN ICU AND EMERGENCY SECTIONS IN ONE OF EDUCATIONAL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHORBANI TAZHANDARE SHAFIE; IMANI FOOLADI ABBAS ALI; NOURANI MOHAMMAD REZA

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Studies in Medical Sciences Year:2013 | Volume:24 | Issue:2 Page(s): 110-120

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

655

Download:

Cites:

Information Journal Paper

Title

PREVALENCE OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS BY DISK DIFFUSION AND MOLECULAR METHODS IN ICU AND EMERGENCY SECTIONS IN ONE OF EDUCATIONAL

Author(s)

GHORBANI TAZHANDARE SHAFIE | IMANI FOOLADI ABBAS ALI | NOURANI MOHAMMAD REZA | Issue Writer Certificate

Keywords

STAPHYLOCOCCUS AUREUSQ2

ANTIBIOTIC RESISTANTQ2

METHICILLINQ2

MRSAQ2

Abstract

Background: METHICILLIN-Resistant STAPHYLOCOCCUS AUREUS (MSRA) is one of the main causes of serious infections in Iran and other countries. Treatment of these infections has become more difficult because of resistance to most antibiotics. The aim of this study was to determine the incidence of METHICILLIN-resistance gene in S. aureus isolates and antibiotic susceptibility to oxacillin and other antibiotics were performed by Disc Diffusion Agar (DDA).Materials and Methods: This study was carried out on SA isolates (n=73) collected from the clinical samples at during 2011 in ICU and Emeregency sections. All S. aureus isolates was identified after performing by routine laboratory procedures including, Gram stain, catalase test, DNase production, coagulase activity and mannitol salt fermentation. In this study used to identify Phenotypic by Disc Diffusion Agar (DDA) and for identify genotypic two pairs of primers designed by AlleleID software (version 6) on the basis of published DNA sequences of the S. aureus gene. Synthesis of primers was carry out by Cinnagen Company, Iran. PCR reaction of mecA with designed primers was optimized and incident of mecA was studied in clinical samples in ICU and Emergency sections.Results: The PCR analysis of 73 S. aureus isolates originated from patients for mecA showed 65 (89%) cases were positive. But only 54 (74%) isolates were METHICILLIN resistant when we used the DDA method.Conclusion: As a conclusion, the resistance to METHICILLIN of S. aureus in Baqiyatallah hospitals has reached to 89% and more than 95% of them were multidrug resistance.

Cites

No record.

References

No record.

Cite

APA: Copy

GHORBANI TAZHANDARE, SHAFIE, IMANI FOOLADI, ABBAS ALI, & NOURANI, MOHAMMAD REZA. (2013). PREVALENCE OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS BY DISK DIFFUSION AND MOLECULAR METHODS IN ICU AND EMERGENCY SECTIONS IN ONE OF EDUCATIONAL. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), 24(2), 110-120. SID. https://sid.ir/paper/63999/en

Vancouver: Copy

GHORBANI TAZHANDARE SHAFIE, IMANI FOOLADI ABBAS ALI, NOURANI MOHAMMAD REZA. PREVALENCE OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS BY DISK DIFFUSION AND MOLECULAR METHODS IN ICU AND EMERGENCY SECTIONS IN ONE OF EDUCATIONAL. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES)[Internet]. 2013;24(2):110-120. Available from: https://sid.ir/paper/63999/en

IEEE: Copy

SHAFIE GHORBANI TAZHANDARE, ABBAS ALI IMANI FOOLADI, and MOHAMMAD REZA NOURANI, “PREVALENCE OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS BY DISK DIFFUSION AND MOLECULAR METHODS IN ICU AND EMERGENCY SECTIONS IN ONE OF EDUCATIONAL,” STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), vol. 24, no. 2, pp. 110–120, 2013, [Online]. Available: https://sid.ir/paper/63999/en

Related Journal Papers