COMPARISON OF CULTURE AND MULTIPLEX PCR TECHNIQUE FOR DETECTION OF BRUCELLA ABORTUS AND BRUCELLA MELITENSIS FROM HUMAN BLOOD SAMPLES

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MIRNEJAD REZA; VAHDATI ALI REZA; AHMADI ALI; MORTAZAVI SEIED MOJTABA; PIRANFAR VAHHAB

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Journal: Zahedan Journal of Research in Medical Sciences Year:2013 | Volume:15 | Issue:12 Page(s): 5-8

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Title

COMPARISON OF CULTURE AND MULTIPLEX PCR TECHNIQUE FOR DETECTION OF BRUCELLA ABORTUS AND BRUCELLA MELITENSIS FROM HUMAN BLOOD SAMPLES

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Keywords

BRUCELLA ABORTUS

BRUCELLA MELITENSIS

MULTIPLEX PCR

CULTURE METHOD

Abstract

Background: To compare CULTURE METHODs with MULTIPLEX PCR technique for identification of BRUCELLA ABORTUS and BRUCELLA MELITENSIS from suspicious patients with clinical history of brucellosis and positive serological test (Rose Bengal test and serum agglutination test).Materials and Methods: In this study, 160 blood samples from patients suspected of Brucellosis with high serum titers of 1/80 were studied. All samples were cultured in Brucella-specific media. Brucella species were identified by using microbiological methods. DNA was extracted with Phenol-chloroform DNA extraction method. IS711 was amplified simultaneously using three specific primers and obtained patterns were analyzed.Results: From 160 samples, 47.5% (76) were culture positive cases from which 43 cases were B. melitensis and 33 were B. abortus With the PCR technique 108 were detected positive from which 45.3% were B. abortus and 54.6% were B. melitensis. It should be noted that all 76 samples with positive culture were also identified by PCR.Conclusion: Generally, use of the molecular technique MULTIPLEX PCR in addition to increased speed and accuracy and less false results than bacterial CULTURE METHOD, is able to identify different species of brucella. This will facilitate the treatment process.

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APA: Copy

MIRNEJAD, REZA, VAHDATI, ALI REZA, AHMADI, ALI, MORTAZAVI, SEIED MOJTABA, & PIRANFAR, VAHHAB. (2013). COMPARISON OF CULTURE AND MULTIPLEX PCR TECHNIQUE FOR DETECTION OF BRUCELLA ABORTUS AND BRUCELLA MELITENSIS FROM HUMAN BLOOD SAMPLES. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 15(12), 5-8. SID. https://sid.ir/paper/642517/en

Vancouver: Copy

MIRNEJAD REZA, VAHDATI ALI REZA, AHMADI ALI, MORTAZAVI SEIED MOJTABA, PIRANFAR VAHHAB. COMPARISON OF CULTURE AND MULTIPLEX PCR TECHNIQUE FOR DETECTION OF BRUCELLA ABORTUS AND BRUCELLA MELITENSIS FROM HUMAN BLOOD SAMPLES. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2013;15(12):5-8. Available from: https://sid.ir/paper/642517/en

IEEE: Copy

REZA MIRNEJAD, ALI REZA VAHDATI, ALI AHMADI, SEIED MOJTABA MORTAZAVI, and VAHHAB PIRANFAR, “COMPARISON OF CULTURE AND MULTIPLEX PCR TECHNIQUE FOR DETECTION OF BRUCELLA ABORTUS AND BRUCELLA MELITENSIS FROM HUMAN BLOOD SAMPLES,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 15, no. 12, pp. 5–8, 2013, [Online]. Available: https://sid.ir/paper/642517/en

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