EFFECT OF EXTENDER AND EQUILIBRATION TIME ON POST THAW MOTILITY AND CHROMATIN STRUCTURE OF BUFFALO BULL (BUBALUS BUBALIS) SPERMATOZOA

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SHAHVERDI ABDOLHOSSAIN; RASTEGARNIA ABDOLREZA; REZAEI TOPRAGGALEH TOHID

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Paper Information

Journal: Cell Journal (Yakhteh) Year:2014 | Volume:16 | Issue:3 (63) Page(s): 279-288

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Title

EFFECT OF EXTENDER AND EQUILIBRATION TIME ON POST THAW MOTILITY AND CHROMATIN STRUCTURE OF BUFFALO BULL (BUBALUS BUBALIS) SPERMATOZOA

Author(s)

SHAHVERDI ABDOLHOSSAIN | RASTEGARNIA ABDOLREZA | REZAEI TOPRAGGALEH TOHID | Issue Writer Certificate

Keywords

BUFFALO

SPERM

CRYOPRESERVATION

EXTENDER

CHROMATIN

Abstract

Objective: The aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two EXTENDERs (tris or BioxcellÒ) on CRYOPRESERVATION of BUFFALO semen.Materials and Methods: In this experimental study, split pooled ejaculates (n=4), possessing more than 70% visual SPERM motility were divided in two aliquots and diluted in BioxcellÒ and tris-citric egg yolk (TCE) EXTENDERs. Semen was cooled to 4oC within 2 hours, equilibrated at 4oC for 2, 4, 8 and 16 hours, then transferred into 0.5 ml French straws, and frozen in a programmable cell freezer before being plunged into liquid nitrogen. Postthaw motility characteristics, plasma membrane integrity, acrosome morphology and DNA integrity of the BUFFALO SPERM were studied after thawing.Results: There were signiﬁcant interactions between equilibration times and EXTENDERs for SPERM motility and membrane integrity. Post thaw SPERM motility (PMOT), progressive motile SPERMatozoa (PROG), plasma membrane integrity (PMI) and normal apical ridge (NAR) measures were lower for SPERM equilibrated for 2 hours in both TCE and BioxcellÒ EXTENDER compared to others equilibration times. PMOT, PMI and NAR for SPERM equilibrated for 4, 8 and 16 hours showed no significant differences in either EXTENDER, although PROG measures were superior in BioxcellÒ compared to TCE at all equilibration times (p<0.05). Kinematic parameters such as average path velocity, curvilinear velocity and linearity in the BioxcellÒ EXTENDER were superior to those in the TCE EXTENDER studied. In contrast to motility and viability, the DNA integrity of post thaw SPERMatozoa remained unaffected by different equilibration times.Conclusion: Equilibration time is necessary for preservation of the motility and integrity of BUFFALO SPERM membranes. Equilibration times of over than 2 hours resulted in the greatest preservation of total semen parameters during CRYOPRESERVATION. There were no signiﬁcant interactions between equilibration times over 4 hours and type of EXTENDER which lead to greater post thaw SPERM survival.

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APA: Copy

SHAHVERDI, ABDOLHOSSAIN, RASTEGARNIA, ABDOLREZA, & REZAEI TOPRAGGALEH, TOHID. (2014). EFFECT OF EXTENDER AND EQUILIBRATION TIME ON POST THAW MOTILITY AND CHROMATIN STRUCTURE OF BUFFALO BULL (BUBALUS BUBALIS) SPERMATOZOA. CELL JOURNAL (YAKHTEH), 16(3 (63)), 279-288. SID. https://sid.ir/paper/645089/en

Vancouver: Copy

SHAHVERDI ABDOLHOSSAIN, RASTEGARNIA ABDOLREZA, REZAEI TOPRAGGALEH TOHID. EFFECT OF EXTENDER AND EQUILIBRATION TIME ON POST THAW MOTILITY AND CHROMATIN STRUCTURE OF BUFFALO BULL (BUBALUS BUBALIS) SPERMATOZOA. CELL JOURNAL (YAKHTEH)[Internet]. 2014;16(3 (63)):279-288. Available from: https://sid.ir/paper/645089/en

IEEE: Copy

ABDOLHOSSAIN SHAHVERDI, ABDOLREZA RASTEGARNIA, and TOHID REZAEI TOPRAGGALEH, “EFFECT OF EXTENDER AND EQUILIBRATION TIME ON POST THAW MOTILITY AND CHROMATIN STRUCTURE OF BUFFALO BULL (BUBALUS BUBALIS) SPERMATOZOA,” CELL JOURNAL (YAKHTEH), vol. 16, no. 3 (63), pp. 279–288, 2014, [Online]. Available: https://sid.ir/paper/645089/en

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