GENERATION AND CHARACTERIZATION OF ANTI-CD34 MONOCLONAL ANTIBODIES THAT REACT WITH HEMATOPOIETIC STEM CELLS (SHORT COMMUNICATION)

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AGHEBATI MALEKI LEILI; MAJIDI JAFAR; BARADARAN BEHZAD; MOVASSAGHPOUR ALIAKBAR; ABDOLALIZADEH JALAL

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Journal: Cell Journal (Yakhteh) Year:2014 | Volume:16 | Issue:3 (63) Page(s): 361-366

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Title

GENERATION AND CHARACTERIZATION OF ANTI-CD34 MONOCLONAL ANTIBODIES THAT REACT WITH HEMATOPOIETIC STEM CELLS (SHORT COMMUNICATION)

Author(s)

Keywords

MONOCLONAL ANTIBODY

CD34

HEMATOPOIETIC STEM CELLS

ISOLATION

Abstract

CD34 is a type I membrane protein with a molecular mass of approximately 110 kDa. This antigen is associated with human hematopoietic progenitor cells and is a differentiation stage-specific leukocyte antigen. In this study we have generated and characterized monoclonal antibodies (mAbs) directed against a CD34 marker. Mice were immunized with two keyhole lympet hemocyanin (KLH) -conjugated CD34 peptides. Fused cells were grown in hypoxanthine, aminopterine and thymidine (HAT) selective medium and cloned by the limiting dilution (L.D) method. Several monoclones were isolated by three rounds of limited dilutions. From these, we chose stable clones that presented sustained antibody production for subsequent characterization. Antibodies were tested for their reactivity and specificity to recognize the CD34 peptides and further screened by enzyme-linked immunosorbent assay (ELISA) and Western blotting analyses. One of the mAbs (3D5) was strongly reactive against the CD34 peptide and with native CD34 from human umbilical cord blood cells (UCB) in ELISA and Western blotting analyses. The results have shown that this antibody is highly specific and functional in biomedical applications such as ELISA and Western blot assays. This monoclonal antibodies (mAb) can be a useful tool for ISOLATION and purification of human HEMATOPOIETIC STEM CELLS (HSCs).

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APA: Copy

AGHEBATI MALEKI, LEILI, MAJIDI, JAFAR, BARADARAN, BEHZAD, MOVASSAGHPOUR, ALIAKBAR, & ABDOLALIZADEH, JALAL. (2014). GENERATION AND CHARACTERIZATION OF ANTI-CD34 MONOCLONAL ANTIBODIES THAT REACT WITH HEMATOPOIETIC STEM CELLS (SHORT COMMUNICATION). CELL JOURNAL (YAKHTEH), 16(3 (63)), 361-366. SID. https://sid.ir/paper/645095/en

Vancouver: Copy

AGHEBATI MALEKI LEILI, MAJIDI JAFAR, BARADARAN BEHZAD, MOVASSAGHPOUR ALIAKBAR, ABDOLALIZADEH JALAL. GENERATION AND CHARACTERIZATION OF ANTI-CD34 MONOCLONAL ANTIBODIES THAT REACT WITH HEMATOPOIETIC STEM CELLS (SHORT COMMUNICATION). CELL JOURNAL (YAKHTEH)[Internet]. 2014;16(3 (63)):361-366. Available from: https://sid.ir/paper/645095/en

IEEE: Copy

LEILI AGHEBATI MALEKI, JAFAR MAJIDI, BEHZAD BARADARAN, ALIAKBAR MOVASSAGHPOUR, and JALAL ABDOLALIZADEH, “GENERATION AND CHARACTERIZATION OF ANTI-CD34 MONOCLONAL ANTIBODIES THAT REACT WITH HEMATOPOIETIC STEM CELLS (SHORT COMMUNICATION),” CELL JOURNAL (YAKHTEH), vol. 16, no. 3 (63), pp. 361–366, 2014, [Online]. Available: https://sid.ir/paper/645095/en

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