THE IMPORTANT ROLE OF FLT3-L IN EX VIVO EXPANSION OF HEMATOPOIETIC STEM CELLS FOLLOWING CO-CULTURE WITH MESENCHYMAL STEM CELLS

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OUBARI FARHAD; AMIRIZADEH NASER; MOHAMMADPOUR HEMN; NAKHLESTANI MOZHDEH; NIKOUGOFTAR ZARIF MAHIN

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Journal Paper

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Journal: Cell Journal (Yakhteh) Year:2015 | Volume:17 | Issue:2 Page(s): 201-210

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Title

THE IMPORTANT ROLE OF FLT3-L IN EX VIVO EXPANSION OF HEMATOPOIETIC STEM CELLS FOLLOWING CO-CULTURE WITH MESENCHYMAL STEM CELLS

Author(s)

Keywords

FMS-RELATED TYROSINE KINASE 3 LIGAND

HEMATOPOIETIC STEM CELLS

MESENCHYMAL STEM CELLS

EXPANSION

Abstract

Objective: HEMATOPOIETIC STEM CELLS (HSCs) transplantation using umbilical cord blood (UCB) has improved during the last decade. Because of cell limitations, several studies focused on the ex vivo EXPANSION of HSCs. Numerous investigations were performed to introduce the best cytokine cocktails for HSC EXPANSION The majority used the FMS-RELATED TYROSINE KINASE 3 LIGAND (FLT3-L) as a critical component. According to FLT3-L biology, in this study we have investigated the hypothesis that FLT3-L only effectively induces HSCs EXPANSION in the presence of a mesenchymal stem cell (MSC) feeder.Materials and Methods: In this experimental study, HSCs and MSCs were isolated from UCB and placenta, respectively. HSCs were cultured in different culture conditions in the presence and absence of MSC feeder and cytokines. After ten days of culture, total nucleated cell count (TNC), cluster of differentiation 34+ (CD34+) cell count, colony forming unit assay (CFU), long-term culture initiating cell (LTC-IC), homeobox protein B4 (HoxB4) mRNA and surface CD49d expression were evaluated. The fold increase for some culture conditions was compared by the t test.Results: HSCs expanded in the presence of cytokines and MSCs feeder. The rate of EXPANSION in the co-culture condition was two-fold more than culture with cytokines (P<0.05). FLT3-L could expand HSCs in the co-culture condition at a level of 20-fold equal to the presence of stem cell factor (SCF), thrombopoietin (TPO) and FLT3-L without feeder cells. The number of extracted colonies from LTC-IC and CD49d expression compared with a cytokine cocktail condition meaningfully increased (P<0.05).Conclusion: FLT3-L co-culture with MSCs can induce high yield EXPANSION of HSCs and be a substitute for the universal cocktail of SCF, TPO and FLT3-L in feeder-free culture.

Cites

Mild hypoxia and human bone marrow mesenchymal stem cells synergistically enhance expansion and homing capacity of human cord blood CD34+ stem cells

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APA: Copy

OUBARI, FARHAD, AMIRIZADEH, NASER, MOHAMMADPOUR, HEMN, NAKHLESTANI, MOZHDEH, & NIKOUGOFTAR ZARIF, MAHIN. (2015). THE IMPORTANT ROLE OF FLT3-L IN EX VIVO EXPANSION OF HEMATOPOIETIC STEM CELLS FOLLOWING CO-CULTURE WITH MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), 17(2), 201-210. SID. https://sid.ir/paper/648992/en

Vancouver: Copy

OUBARI FARHAD, AMIRIZADEH NASER, MOHAMMADPOUR HEMN, NAKHLESTANI MOZHDEH, NIKOUGOFTAR ZARIF MAHIN. THE IMPORTANT ROLE OF FLT3-L IN EX VIVO EXPANSION OF HEMATOPOIETIC STEM CELLS FOLLOWING CO-CULTURE WITH MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2015;17(2):201-210. Available from: https://sid.ir/paper/648992/en

IEEE: Copy

FARHAD OUBARI, NASER AMIRIZADEH, HEMN MOHAMMADPOUR, MOZHDEH NAKHLESTANI, and MAHIN NIKOUGOFTAR ZARIF, “THE IMPORTANT ROLE OF FLT3-L IN EX VIVO EXPANSION OF HEMATOPOIETIC STEM CELLS FOLLOWING CO-CULTURE WITH MESENCHYMAL STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 17, no. 2, pp. 201–210, 2015, [Online]. Available: https://sid.ir/paper/648992/en

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