MORPHOLOGICAL STUDY OF ISOLATED OVARIAN PREANTRAL FOLLICLES USING FIBRIN GEL PLUS PLATELET LYSATE AFTER SUBCUTANEOUS TRANSPLANTATION

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RAJABZADEH ALI REZA; EIMANI HOSSEIN; MOHSENI KOOCHESFAHANI HOMA; SHAHVARDI ABDOL HOSSEIN; FATHI ROUHOLLAH

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Journal: Cell Journal (Yakhteh) Year:2015 | Volume:17 | Issue:1 (65) Page(s): 145-152

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Title

MORPHOLOGICAL STUDY OF ISOLATED OVARIAN PREANTRAL FOLLICLES USING FIBRIN GEL PLUS PLATELET LYSATE AFTER SUBCUTANEOUS TRANSPLANTATION

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Keywords

TRANSPLANTATION

FOLLICLE

OVARY

Abstract

Objective: Ovarian and FOLLICLE TRANSPLANTATION may preserve fertility in young cancer survivors. In this study, we have transplanted preantral FOLLICLEs using fibrin gel as a carrier and fibrin gel supplemented with platelet lysate (PL) as a rich source of angiogenic and growth factors. The purpose of this study was to evaluate the role of fibrin gel and PL in FOLLICLE TRANSPLANTATION.Materials and Methods: In this experimental study, ovaries were taken from 14-dayold Naval Medical Research Institute (NMRI) mice. Preantral FOLLICLEs were dissected from the ovaries and encapsulated into fibrin gel supplemented with 5, 10, 15 or 20% PL, then transplanted back into the same donor mice. Fibrin gels supplemented with PL that contained preantral FOLLICLEs were placed in a subcutaneous pocket in the back of the neck of the recipient, donor mouse (the same mouse that FOLLICLEs were collected). After 14 days the grafts were processed and embedded in paraffin blocks, then serially sectioned for histological evaluation. We counted the FOLLICLEs and classified them according to stage (preantral or antral). Data were presented as mean±standard error of mean (SEM) and analysed by analysis of variance (ANOVA) and the Kruskal-Wallistest.Results: The mean percentage of recovered FOLLICLEs encapsulated and transplanted in each group were 33.30±2.47 (fibrin gel), 31.96±1.90 (fibrin gel+5% PL), 34.02±2.44 (fibrin gel+10% PL), 48.31±2.06 (fibrin gel+15% PL) and 17.60±2.79 (fibrin gel+20% PL). There was a significant increase in the recovery rate of grafted FOLLICLEs with fibrin gel+15% PL (48.31%; p<0.001). The percentage of preantral FOLLICLEs showed no significant difference in all groups (p<0.05). The percentage of antral FOLLICLEs showed a significant decrease in FOLLICLEs grafted with fibrin gel+20% PL when compared to the other groups (11.77%; p<0.005) but no significant difference was observed in the other groups.Conclusion: The use of PL in FOLLICLE TRANSPLANTATION can improve ovarian follicular survival rate.

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APA: Copy

RAJABZADEH, ALI REZA, EIMANI, HOSSEIN, MOHSENI KOOCHESFAHANI, HOMA, SHAHVARDI, ABDOL HOSSEIN, & FATHI, ROUHOLLAH. (2015). MORPHOLOGICAL STUDY OF ISOLATED OVARIAN PREANTRAL FOLLICLES USING FIBRIN GEL PLUS PLATELET LYSATE AFTER SUBCUTANEOUS TRANSPLANTATION. CELL JOURNAL (YAKHTEH), 17(1 (65)), 145-152. SID. https://sid.ir/paper/649013/en

Vancouver: Copy

RAJABZADEH ALI REZA, EIMANI HOSSEIN, MOHSENI KOOCHESFAHANI HOMA, SHAHVARDI ABDOL HOSSEIN, FATHI ROUHOLLAH. MORPHOLOGICAL STUDY OF ISOLATED OVARIAN PREANTRAL FOLLICLES USING FIBRIN GEL PLUS PLATELET LYSATE AFTER SUBCUTANEOUS TRANSPLANTATION. CELL JOURNAL (YAKHTEH)[Internet]. 2015;17(1 (65)):145-152. Available from: https://sid.ir/paper/649013/en

IEEE: Copy

ALI REZA RAJABZADEH, HOSSEIN EIMANI, HOMA MOHSENI KOOCHESFAHANI, ABDOL HOSSEIN SHAHVARDI, and ROUHOLLAH FATHI, “MORPHOLOGICAL STUDY OF ISOLATED OVARIAN PREANTRAL FOLLICLES USING FIBRIN GEL PLUS PLATELET LYSATE AFTER SUBCUTANEOUS TRANSPLANTATION,” CELL JOURNAL (YAKHTEH), vol. 17, no. 1 (65), pp. 145–152, 2015, [Online]. Available: https://sid.ir/paper/649013/en

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