FEASIBILITY STUDY OF ANTI-NEUROFILAMENT ANTIBODIES DETECTION BY INDIRECT QUENCHING FLUOROIMMUNOASSAY

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NAKHAEE ALIREZA; MESSRIPOUR MANUCHEHR; KHOSRAVI ALIREZA; SALAMI SAEEDEH; ALIAHMMAD GHOLAMHOSEIN

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Journal: Zahedan Journal of Research in Medical Sciences Year:2014 | Volume:16 | Issue:4 Page(s): 11-14

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Title

FEASIBILITY STUDY OF ANTI-NEUROFILAMENT ANTIBODIES DETECTION BY INDIRECT QUENCHING FLUOROIMMUNOASSAY

Author(s)

Keywords

NEUROFILAMENT

ANTI-NEUROFILAMENT

FLUOROIMMUNOASSAY

NEURODEGENERATIVE DISEASE

Abstract

Background: NEUROFILAMENTs (NFs) are the main constitutes of intermediate filaments in neurons. They are composed of three subunits with heavey, medium and low molecular weight. Anti-NEUROFILAMENT antibodies exist in serum of patients with some NEURODEGENERATIVE DISEASEs.Materials and Methods: A FLUOROIMMUNOASSAY has been developed for determining of antibodies against NEUROFILAMENTs, using an anti-fluorescein serum and fluorescein-labeled NFs. Antibodies raised against bovine spinal cord NFs in rabbit and the labeled NFs are incubated with anti-fluorescein serum at room temperature.Results: At high levels, binding of anti-NEUROFILAMENTs (anti-NFs) to labeled NFs prevented subsequent binding of the anti-fluorescein to fluorescein groups, resulting in little change in the signals of the label. Conversely, at low level of anti-NFs the free fraction of the labeled NFs is available to be bound by anti-fluorescein, which markedly reduced fluorescence intensity of label. Thus, the fluorescence intensity of assay mixture directly reflects the amount of anti-NFs antibodies in the serum.Conclusion: It is concluded that the availability of fluorescein-labeled NFs and antibody directed against fluorescein group permit measurement of anti-NFs antibodies in serum of neurodegenerative patients.

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APA: Copy

NAKHAEE, ALIREZA, MESSRIPOUR, MANUCHEHR, KHOSRAVI, ALIREZA, SALAMI, SAEEDEH, & ALIAHMMAD, GHOLAMHOSEIN. (2014). FEASIBILITY STUDY OF ANTI-NEUROFILAMENT ANTIBODIES DETECTION BY INDIRECT QUENCHING FLUOROIMMUNOASSAY. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 16(4), 11-14. SID. https://sid.ir/paper/649676/en

Vancouver: Copy

NAKHAEE ALIREZA, MESSRIPOUR MANUCHEHR, KHOSRAVI ALIREZA, SALAMI SAEEDEH, ALIAHMMAD GHOLAMHOSEIN. FEASIBILITY STUDY OF ANTI-NEUROFILAMENT ANTIBODIES DETECTION BY INDIRECT QUENCHING FLUOROIMMUNOASSAY. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2014;16(4):11-14. Available from: https://sid.ir/paper/649676/en

IEEE: Copy

ALIREZA NAKHAEE, MANUCHEHR MESSRIPOUR, ALIREZA KHOSRAVI, SAEEDEH SALAMI, and GHOLAMHOSEIN ALIAHMMAD, “FEASIBILITY STUDY OF ANTI-NEUROFILAMENT ANTIBODIES DETECTION BY INDIRECT QUENCHING FLUOROIMMUNOASSAY,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 16, no. 4, pp. 11–14, 2014, [Online]. Available: https://sid.ir/paper/649676/en

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