مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Cites:

1

Information Journal Paper

Title

IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL CORD BLOOD CD133+ CELLS INTO INSULIN PRODUCING CELLS IN CO-CULTURE WITH RAT PANCREATIC MESENCHYMAL STEM CELLS

Pages

  211-220

Abstract

 Objective: Pancreatic stroma plays an important role in the induction of pancreatic cells by the use of close range signaling. In this respect, we presume that pancreatic mesenchymal cells (PMCs) as a fundamental factor of the stromal niche may have an effective role in differentiation of UMBILICAL CORD blood cluster of differentiation 133+ (UCB-CD133+) cells into newly-formed b-cells in vitro.Materials and Methods: This study is an experimental research. The UCB-CD133+ cells were purified by magnetic activated cell sorting (MACS) and differentiated into insulin producing cells (IPCs) in co-culture, both directly and indirectly with rat PMCs. Immunocytochemistry and enzyme linked immune sorbent assay (ELISA) were used to determine expression and production of insulin and C-peptide at the protein level.Results: Our results demonstrated that UCB-CD133+ differentiated into IPCs. Cells in islet-like clusters with (out) co-cultured with rat pancreatic stromal cells produced insulin and C-peptide and released them into the culture medium at the end of the induction protocol. However they did not respond well to glucose challenges.Conclusion: Rat PMCs possibly affect differentiation of UCB-CD133+cells into IPCs by increasing the number of immature b-cells.

Cites

References

Cite

APA: Copy

SAHRANESHIN SAMANI, FAZEL, EBRAHIMI, MARZIEH, ZANDIEH, TAHEREH, KHOSHCHEHREH, REYHANEH, BAGHABAN ESLAMINEJAD, MOHAMADREZA, AGHDAMI, NASSER, & BAHARVAND, HOSSEIN. (2015). IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL CORD BLOOD CD133+ CELLS INTO INSULIN PRODUCING CELLS IN CO-CULTURE WITH RAT PANCREATIC MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), 17(2), 211-220. SID. https://sid.ir/paper/650685/en

Vancouver: Copy

SAHRANESHIN SAMANI FAZEL, EBRAHIMI MARZIEH, ZANDIEH TAHEREH, KHOSHCHEHREH REYHANEH, BAGHABAN ESLAMINEJAD MOHAMADREZA, AGHDAMI NASSER, BAHARVAND HOSSEIN. IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL CORD BLOOD CD133+ CELLS INTO INSULIN PRODUCING CELLS IN CO-CULTURE WITH RAT PANCREATIC MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2015;17(2):211-220. Available from: https://sid.ir/paper/650685/en

IEEE: Copy

FAZEL SAHRANESHIN SAMANI, MARZIEH EBRAHIMI, TAHEREH ZANDIEH, REYHANEH KHOSHCHEHREH, MOHAMADREZA BAGHABAN ESLAMINEJAD, NASSER AGHDAMI, and HOSSEIN BAHARVAND, “IN VITRO DIFFERENTIATION OF HUMAN UMBILICAL CORD BLOOD CD133+ CELLS INTO INSULIN PRODUCING CELLS IN CO-CULTURE WITH RAT PANCREATIC MESENCHYMAL STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 17, no. 2, pp. 211–220, 2015, [Online]. Available: https://sid.ir/paper/650685/en

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