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Information Journal Paper

Title

CYSTEINE: A NOVEL NEURAL INDUCER FOR RAT BONE MARROW MESENCHYMAL STEM CELLS

Pages

  195-202

Abstract

 Objective: MESENCHYMAL STEM CELLs (MSCs) can differentiate into various cell types. Since CYSTEINE has structural similarities to neuronal inducers b-mercaptoethanol and glutathione, we examined its effect on NEURAL INDUCTION of rat bone marrow MSCs.Materials and Methods: In this experimental study, cells were treated in a medium containing 1mM CYSTEINE for 24 hours prior to treatment with neuron inducing medium containing 10 mM CYSTEINE for 1, 2 and 3 hours. Cell viability and morphology were assessed by 3- (4, 5-dimethylthiazol-2-Yl) -2, 5-diphenyltetrazolium bromide (MTT) assay and, Hoechst, propidium iodide and acridine orange staining respectively. Expression of NESTIN and b-Tubulin III genes, as neural cell-specific markers, was studied reverse transcription polymerase chain reaction (RT-PCR). The data was statistically analyzed using One-Way ANOVA and Tukey's test and p<0.05 was considered significant.Results: After 3 hours of treatment, neuron like morphology with a considerable expression of NESTIN and b-Tubulin III genes was apparent. The mean cell viability was not significantly different at 1, 2 and 3 hours following induction, compared with the control cells.Conclusion: CYSTEINE can induce neural features in rat bone marrow MSCs without reducing cell viability. Therefore, it can be considered as a safer alternative to toxic neural inducer agents such as b-mercaptoethanol.

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    Cite

    APA: Copy

    SOLEIMANI MEHRANJANI, MALEK, & FALAHAT CHIAN, MILAD. (2014). CYSTEINE: A NOVEL NEURAL INDUCER FOR RAT BONE MARROW MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), 16(2 (62)), 195-202. SID. https://sid.ir/paper/658362/en

    Vancouver: Copy

    SOLEIMANI MEHRANJANI MALEK, FALAHAT CHIAN MILAD. CYSTEINE: A NOVEL NEURAL INDUCER FOR RAT BONE MARROW MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2014;16(2 (62)):195-202. Available from: https://sid.ir/paper/658362/en

    IEEE: Copy

    MALEK SOLEIMANI MEHRANJANI, and MILAD FALAHAT CHIAN, “CYSTEINE: A NOVEL NEURAL INDUCER FOR RAT BONE MARROW MESENCHYMAL STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 16, no. 2 (62), pp. 195–202, 2014, [Online]. Available: https://sid.ir/paper/658362/en

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