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Cites:

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Information Journal Paper

Title

MIGRATION OF BONE MARROW-DERIVED VERY SMALL EMBRYONIC-LIKE STEM CELLS TOWARD AN INJURED SPINAL CORD

Pages

  639-647

Abstract

 Objective: Bone marrow (BM) is one of the major hematopoietic organs in postnatal life that consists of a heterogeneous population of stem cells which have been previously described. Recently, a rare population of stem cells that are called very small embryonic-like (VSEL) stem cells has been found in the BM. These cells express several developmental markers of pluripotent stem cells and can be mobilized into peripheral blood (PB) in response to tissue injury. In this study we have attempted to investigate the ability of these cells to migrate toward an injured spinal cord after transplantation through the tail vein in a rat model.Materials and Methods: In this experimental study, VSELs were isolated from total BM cells using a fluorescent activated cell sorting (FACS) system and sca1 and stage specific embryonic antigen (SSEA-1) antibodies. After isolation, VSELs were cultured for 7 days on C2C12 as the feeder layer. Then, VSELs were labeled with 1, 1´-dioctadecyl-3, 3, 3´, 3´- tetramethylindocarbocyanine perchlorate (DiI) and transplanted into the rat SPINAL CORD INJURY (SCI) model via the tail vein. Finally, we sought to determine the presence of VSELs in the lesion site.Results: We isolated a high number of VSELs from the BM. After cultivation, the VSELs colonies were positive for SSEA-1, Oct4 and Sca1. At one month after transplantation, real-time polymerase chain reaction analysis confirmed a significantly increased expression level of Oct4 and SSEA-1 positive cells at the injury site.Conclusion: VSELs have the capability to migrate and localize in an injured spinal cord after transplantation.

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    APA: Copy

    GOLIPOOR, ZOLEIKHA, MEHRAEIN, FERESHTEH, ZAFARI, FARIBA, ALIZADEH, AKRAM, ABABZADEH, SHIMA, & BAAZM, MARYAM. (2016). MIGRATION OF BONE MARROW-DERIVED VERY SMALL EMBRYONIC-LIKE STEM CELLS TOWARD AN INJURED SPINAL CORD. CELL JOURNAL (YAKHTEH), 17(4), 639-647. SID. https://sid.ir/paper/669573/en

    Vancouver: Copy

    GOLIPOOR ZOLEIKHA, MEHRAEIN FERESHTEH, ZAFARI FARIBA, ALIZADEH AKRAM, ABABZADEH SHIMA, BAAZM MARYAM. MIGRATION OF BONE MARROW-DERIVED VERY SMALL EMBRYONIC-LIKE STEM CELLS TOWARD AN INJURED SPINAL CORD. CELL JOURNAL (YAKHTEH)[Internet]. 2016;17(4):639-647. Available from: https://sid.ir/paper/669573/en

    IEEE: Copy

    ZOLEIKHA GOLIPOOR, FERESHTEH MEHRAEIN, FARIBA ZAFARI, AKRAM ALIZADEH, SHIMA ABABZADEH, and MARYAM BAAZM, “MIGRATION OF BONE MARROW-DERIVED VERY SMALL EMBRYONIC-LIKE STEM CELLS TOWARD AN INJURED SPINAL CORD,” CELL JOURNAL (YAKHTEH), vol. 17, no. 4, pp. 639–647, 2016, [Online]. Available: https://sid.ir/paper/669573/en

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