مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

246
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

181
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

DECELLULARIZED KIDNEY IN THE PRESENCE OF CHONDROITIN SULFATE AS A NATURAL 3D SCAFFOLD FOR STEM CELLS

Pages

  788-798

Abstract

 Objective (s): Use of biological scaffolds and automating the cells directing process with materials such as growth factors and glycosaminoglycans (GAGs) in a certain path may have beneficial effects in tissue engineering and regenerative medicine in future. In this research, CHONDROITIN SULFATE sodium was used for impregnation of the scaffolds. It is a critical component in EXTRACELLULAR MATRIX and plays an important role in signaling pathway; however, little is known about its role within mammalian development and cell linage specification.Materials and Methods: Due to its porous and appropriate structure and for putting cells in 3D space, the kidney of BALB/c mouse was selected and decellulalized using physical and chemical methods. After decellularization, the scaffold was impregnated in CHONDROITIN SULFATE solution (CS) for 24 hr. Then, 60×105 human adipose‐derived MESENCHYMAL STEM CELLs were seeded on the scaffold to assess their behavior on day 5, 10, 15, 20, and 25.Result: After 48 hr, DAPI staining approved completed decellularized kidney by 1% SDS (sodium dodecyl sulfate). Migration and establishment of a number of cells to the remaining area of the glomerulus was observed. In addition, cell accumulation on the scaffold surface as well as cells migration to the depth of kidney formed an epithelium‐like structure. Up to the day 15, microscopic study of different days of seeding showed the gradual adhesion of large number of cells to the scaffold.Conclusion: Glycosaminoglycan could be a right option for impregnation. It is used for smartification and strengthening of natural scaffolds and induction of some behaviors in stem cells.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    RAFIGHDOUST, ALIREZA, MAHDAVI SHAHRI, NASSER, & BAHARARA, JAVAD. (2015). DECELLULARIZED KIDNEY IN THE PRESENCE OF CHONDROITIN SULFATE AS A NATURAL 3D SCAFFOLD FOR STEM CELLS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 18(8), 788-798. SID. https://sid.ir/paper/672783/en

    Vancouver: Copy

    RAFIGHDOUST ALIREZA, MAHDAVI SHAHRI NASSER, BAHARARA JAVAD. DECELLULARIZED KIDNEY IN THE PRESENCE OF CHONDROITIN SULFATE AS A NATURAL 3D SCAFFOLD FOR STEM CELLS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2015;18(8):788-798. Available from: https://sid.ir/paper/672783/en

    IEEE: Copy

    ALIREZA RAFIGHDOUST, NASSER MAHDAVI SHAHRI, and JAVAD BAHARARA, “DECELLULARIZED KIDNEY IN THE PRESENCE OF CHONDROITIN SULFATE AS A NATURAL 3D SCAFFOLD FOR STEM CELLS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 18, no. 8, pp. 788–798, 2015, [Online]. Available: https://sid.ir/paper/672783/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button