HSA-MIR-590-5P INTERACTION WITH SMAD3 TRANSCRIPT SUPPORTS ITS REGULATORY EFFECT ON THE TGFβ SIGNALING PATHWAY

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JAFARZADEH MEISAM; MOHAMMAD SOLTANI BAHRAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2016 | Volume:18 | Issue:1 (69) Page(s): 7-12

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Title

HSA-MIR-590-5P INTERACTION WITH SMAD3 TRANSCRIPT SUPPORTS ITS REGULATORY EFFECT ON THE TGFβ SIGNALING PATHWAY

Author(s)

JAFARZADEH MEISAM | MOHAMMAD SOLTANI BAHRAM | Issue Writer Certificate

Keywords

HSA-MIR-590-5P

SMAD3

TGFβ

Abstract

Objective: SMAD proteins are the core players of the transforming growth factor-beta (TGFb) signaling pathway, a pathway which is involved in cell proliferation, differentiation and migration. On the other hand, hsa-miRNA-590-5p (miR-590-5p) is known to have a negative regulatory effect on TGFb signaling pathway receptors. Since, RNAhybrid analysis suggested SMAD3 as a bona fide target gene for miR-590, we intended to investigate the effect of miR-590-5p on SMAD3 transcription.Materials and Methods: In this experimental study, miR-590-5p was overexpressed in different cell lines and its increased expression was detected through quantitative reverse transcription-polymerase chain reaction (RT-qPCR). Western blot analysis was then used to investigate the effect of miR-590-5p overexpression on SMAD3 protein level. Next, the direct interaction of miR-590-5p with the 3’-UTR sequence of SMAD3 transcript was investigated using the dual luciferase assay. Finally, flow cytometery was used to investigate the effect of miR-590-5p overexpression on cell cycle progression in HeLa and SW480 cell lines.Results: miR-590-5p was overexpressed in the SW480 cell line and its overexpression resulted in significant reduction of the SMAD3 protein level. Consistently, direct interaction of miR-590-5p with 3’-UTR sequence of SMAD3 was detected. Finally, miR-590-5p overexpression did not show a significant effect on cell cycle progression of Hela and SW480 cell lines.Conclusion: Consistent with previous reports about the negative regulatory effect of miR-590 on TGFb receptors, our data suggest that miR-590-5p also attenuates the TGFb signaling pathway through down-regulation of SMAD3.

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APA: Copy

JAFARZADEH, MEISAM, & MOHAMMAD SOLTANI, BAHRAM. (2016). HSA-MIR-590-5P INTERACTION WITH SMAD3 TRANSCRIPT SUPPORTS ITS REGULATORY EFFECT ON THE TGFβ SIGNALING PATHWAY. CELL JOURNAL (YAKHTEH), 18(1 (69)), 7-12. SID. https://sid.ir/paper/675306/en

Vancouver: Copy

JAFARZADEH MEISAM, MOHAMMAD SOLTANI BAHRAM. HSA-MIR-590-5P INTERACTION WITH SMAD3 TRANSCRIPT SUPPORTS ITS REGULATORY EFFECT ON THE TGFβ SIGNALING PATHWAY. CELL JOURNAL (YAKHTEH)[Internet]. 2016;18(1 (69)):7-12. Available from: https://sid.ir/paper/675306/en

IEEE: Copy

MEISAM JAFARZADEH, and BAHRAM MOHAMMAD SOLTANI, “HSA-MIR-590-5P INTERACTION WITH SMAD3 TRANSCRIPT SUPPORTS ITS REGULATORY EFFECT ON THE TGFβ SIGNALING PATHWAY,” CELL JOURNAL (YAKHTEH), vol. 18, no. 1 (69), pp. 7–12, 2016, [Online]. Available: https://sid.ir/paper/675306/en

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