CONCOMITANT USE OF SEA CUCUMBER ORGANIC EXTRACT AND RADIOTHERAPY ON PROLIFERATION AND APOPTOSIS OF CERVICAL (HELA) CELL LINE

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BAHARARA JAVAD; AMINI ELAHEH; VAZIFEDAN VAHID

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Journal: Zahedan Journal of Research in Medical Sciences Year:2016 | Volume:18 | Issue:4 Page(s): 0-0

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Information Journal Paper

Title

CONCOMITANT USE OF SEA CUCUMBER ORGANIC EXTRACT AND RADIOTHERAPY ON PROLIFERATION AND APOPTOSIS OF CERVICAL (HELA) CELL LINE

Author(s)

BAHARARA JAVAD | AMINI ELAHEH | VAZIFEDAN VAHID | Issue Writer Certificate

Keywords

AQUATIC

PHARMACEUTICAL

RADIOTHERAPY

CERVICAL NEOPLASMS

APOPTOSIS

Abstract

Background: Cervical carcinoma is gynecologic malignancy with conventional treatment modality but drug resistance interferes with current therapeutic methods. Therefore, identification of novelnewmodality with low toxicity has uniquely favorable strategy.Objectives: The aim of this study is evaluation of concomitant use of sea cucumber organic extract and RADIOTHERAPY on ovarian cancer.Materials and Methods: In this in vitro experimental study, HeLa cancer cells were cultured and suspended in RPMI (Roswell Park Memorial Institute) 1640 medium supplemented with 10 % FBS (Fetal Bovine Serum), 1 % antibiotic. Cells were treated with extract at different concentrations (0 to 100 mg/mL) for 24 hours. After determination of suitable concentration, the cells were exposed to 2 Gray gamma radiation in presence of extract for 192 seconds and 66 hours were kept in incubator till anti-proliferative assay were evaluated. To assess APOPTOSIS, flow cytometry with PI (Propodium Iodide) and acridine orange staining were performed.Results: Morphological analysis and results from cytotoxicity assay exhibited that 50 mg/mL of sea cucumber extract alone is considered IC50 and combination of gamma radiation became more valuable in growth inhibition. Also, flow cytometry histogram of treated cells indicated sub-G1 peak demonstrating disturbance in membrane integrity and APOPTOSIS cell death. Fluorescence images have been confirmed APOPTOSIS cell death in treatment groups.Conclusions: These data indicate that sea cucumber extract as novel resource of AQUATIC natural products significantly can inhibit cervical cancer cell growth and synergistic effect of natural extract along with radiation therapy was more effective in anti-cervical cancer therapy.

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APA: Copy

BAHARARA, JAVAD, AMINI, ELAHEH, & VAZIFEDAN, VAHID. (2016). CONCOMITANT USE OF SEA CUCUMBER ORGANIC EXTRACT AND RADIOTHERAPY ON PROLIFERATION AND APOPTOSIS OF CERVICAL (HELA) CELL LINE. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 18(4), 0-0. SID. https://sid.ir/paper/678492/en

Vancouver: Copy

BAHARARA JAVAD, AMINI ELAHEH, VAZIFEDAN VAHID. CONCOMITANT USE OF SEA CUCUMBER ORGANIC EXTRACT AND RADIOTHERAPY ON PROLIFERATION AND APOPTOSIS OF CERVICAL (HELA) CELL LINE. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2016;18(4):0-0. Available from: https://sid.ir/paper/678492/en

IEEE: Copy

JAVAD BAHARARA, ELAHEH AMINI, and VAHID VAZIFEDAN, “CONCOMITANT USE OF SEA CUCUMBER ORGANIC EXTRACT AND RADIOTHERAPY ON PROLIFERATION AND APOPTOSIS OF CERVICAL (HELA) CELL LINE,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 18, no. 4, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/678492/en

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