Hypoglycemic activity of the ethyl acetate extract from Smilax glabra Roxb in mice: Biochemical and histopathological studies

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Mai Nguyen Phuong Thi; Ngo Quang Van; Hong Nguyen Minh Thi; Quach Lien Thi; PYNE STEPHEN G.

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Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2020 | Volume:23 | Issue:12 Page(s): 1558-1564

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Title

Hypoglycemic activity of the ethyl acetate extract from Smilax glabra Roxb in mice: Biochemical and histopathological studies

Author(s)

Keywords

Alpha-glucosidase

Alpha-amylase

Diabetes

Ethyl acetate (EtOAc)-

extract

In vivo model

Smilax glabra Roxb

Abstract

Objective(s): This research was carried out to investigate the hypoglycemic activity of the ethyl acetate (EtOAc) extract from the roots of Smilax glabra Roxb, which strongly exhibit inhibitory activity against α-glucosidase and α-amylase on in vivo type 2 diabetic model. Materials and Methods: Column chromatography combined with crystallization was used to isolate the active fraction and compounds. Chemical structures of the compounds were determined based on the analysis of the spectroscopic data and comparison with the literature data. The α-glucosidase inhibitory activity (AGI) and the α-amylase inhibitory activity (AAI) were determined quantitatively spectrophotometrically using p-nitrophenyl α-D-glucopyranoside and soluble starch as substrates, respectively. The hypoglycemic activity was examined by evaluating its effects on glucose and insulin levels, insulin resistance, and histopathology of the pancreatic islets and livers in diabetic induced mice administrated with nicotinamide-streptozotocin. Results: The EtOAc extract and the bioactive compounds astilbin and 5-O-caffeoylshikimic acid in the extract were isolated and confirmed in structures, AGI, and AAI. The treatment at the doses of 500 and 1000 μ g/kg of body weight reduced blood glucose levels down to the physiological level of the physical controls in the diabetic mice after two weeks (P<0. 05). Moreover, the treatment improved insulin sensitivity. Histopathology analysis showed recovering effects in the size of the pancreatic islets and no damaging effects on the liver after treatment compared with the control group. Conclusion: Our data suggest that the EtOAc extract possesses hypoglycemic activity and has an antidiabetic potential for therapeutic applications.

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APA: Copy

Mai Nguyen, Phuong Thi, Ngo, Quang Van, Hong Nguyen, Minh Thi, Quach, Lien Thi, & PYNE, STEPHEN G.. (2020). Hypoglycemic activity of the ethyl acetate extract from Smilax glabra Roxb in mice: Biochemical and histopathological studies. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 23(12), 1558-1564. SID. https://sid.ir/paper/679568/en

Vancouver: Copy

Mai Nguyen Phuong Thi, Ngo Quang Van, Hong Nguyen Minh Thi, Quach Lien Thi, PYNE STEPHEN G.. Hypoglycemic activity of the ethyl acetate extract from Smilax glabra Roxb in mice: Biochemical and histopathological studies. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2020;23(12):1558-1564. Available from: https://sid.ir/paper/679568/en

IEEE: Copy

Phuong Thi Mai Nguyen, Quang Van Ngo, Minh Thi Hong Nguyen, Lien Thi Quach, and STEPHEN G. PYNE, “Hypoglycemic activity of the ethyl acetate extract from Smilax glabra Roxb in mice: Biochemical and histopathological studies,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 23, no. 12, pp. 1558–1564, 2020, [Online]. Available: https://sid.ir/paper/679568/en

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