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Information Journal Paper

Title

EFFECTS OF EXENDINE-4 ON THE DIFFERENTIATION OF INSULIN PRODUCING CELLS FROM RAT ADIPOSE-DERIVED MESENCHYMAL STEM CELLS

Pages

  720-729

Abstract

 Objective: To evaluate the effect of Exendine-4 (EX-4), a Glucagon-like peptide 1 (GLP-1) receptor agonist, on the DIFFERENTIATION of INSULIN-SECRETING CELLS (IPCs) from rat adipose-derived MESENCHYMAL STEM CELLS (ADMSCs).Materials and Methods: In this experimental study, ADMSCs were isolated from rat ADIPOSE TISSUE and exposed to induction media with or without EX-4. After induction, the existence of IPCs was confirmed by morphology analysis, expression pattern analysis of islet-specific genes (Pdx-1, Glut-2 and Insulin) and insulin synthesis and secretion.Results: IPCs induced in presence of EX-4 were morphologically similar to pancreatic islet-like cells. Expression of Pdx-1, Glut-2 and Insulin genes in EX-4 treated cells was significantly higher than the cells exposed to DIFFERENTIATION media without EX-4. Compared to EX-4 untreated ADMSCs, insulin release from EX-4 treated ADMSCs showed a nearly 2.5 fold (P<0.05) increase when exposed to a high glucose (25 mM) medium. The percentage of insulin positive cells in the EX-4 treated group was approximately 4-fold higher than in the EX-4 untreated ADMSCs.Conclusion: The present study has demonstrated that EX-4 enhances the DIFFERENTIATION of ADMSCs into IPCs. Improvement of this method may help the formation of an unlimited source of cells for transplantation.

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    APA: Copy

    KHORSANDI, LAYASADAT, SAREMY, SADEGH, KHODADADI, ALI, & DEHBASHI, FERESHTEH. (2016). EFFECTS OF EXENDINE-4 ON THE DIFFERENTIATION OF INSULIN PRODUCING CELLS FROM RAT ADIPOSE-DERIVED MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), 17(4), 720-729. SID. https://sid.ir/paper/680384/en

    Vancouver: Copy

    KHORSANDI LAYASADAT, SAREMY SADEGH, KHODADADI ALI, DEHBASHI FERESHTEH. EFFECTS OF EXENDINE-4 ON THE DIFFERENTIATION OF INSULIN PRODUCING CELLS FROM RAT ADIPOSE-DERIVED MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2016;17(4):720-729. Available from: https://sid.ir/paper/680384/en

    IEEE: Copy

    LAYASADAT KHORSANDI, SADEGH SAREMY, ALI KHODADADI, and FERESHTEH DEHBASHI, “EFFECTS OF EXENDINE-4 ON THE DIFFERENTIATION OF INSULIN PRODUCING CELLS FROM RAT ADIPOSE-DERIVED MESENCHYMAL STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 17, no. 4, pp. 720–729, 2016, [Online]. Available: https://sid.ir/paper/680384/en

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