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Cites:

1

Information Journal Paper

Title

Effects of Adipose-Derived Stem Cells and Platelet-Rich Plasma Exosomes on The Inductivity of Hair Dermal Papilla Cells

Pages

  576-583

Abstract

 Objective: Hair Loss is a prevalent medical problem in both men and women. Maintaining the Hair Inductivity potential of human dermal papilla cells (hDPCs) during cell culture is the main issue in hair follicle morphogenesis and regeneration. The present study was conducted to compare the effects of different concentrations of Exosomes derived from human Adipose Stem Cells (hASCs) and Platelet-Rich Plasma (PRP) on the proliferation, migration and expression of alkaline pholphatase (ALP), versican, and smooth muscle alpha-actin (α-SMA) in human DPCs. Materials and Methods: In this experimental study, hDPCs, human hair DPCs and outer root sheet cells (ORSCs) were separated from healthy hair samples. The protocol of Exosome isolation from PRP and hASCs comprises serial low speed centrifugation and ultracentrifugation. The effects of different concentrations of Exosomes (25, 50, 100 μ g/ ml) derived from hASCs and PRP on proliferation (MTS assay), migration (scratch test) and expression of ALP, versican and α-SMA (real time-polymerase chain reaction) in human DPCs were evaluated. Results: The flow cytometry analysis of specific cytoplasmic markers showed expression of versican (77%) and α-SMA (60. 8%) in DPCs and K15 (73. 2%) in ORSCs. According to NanoSight Dynamic Light Scattering, we found the majority of ASCs and PRP-Exosomes (ASC-Exo and PRP-Exo) to be 30-150 nm in size. For 100 μ g/ml of ASCs-Exo, the expressions of ALP, versican and α-SMA proteins increased by a factor of 1. 2, 2 and 3, respectively, compared to the control group. The findings of our experiments illustrated that 100 μ g/ml of ASCs-Exo compared to the same concentration of PRP-Exo significantly promote DPC proliferation and migration in culture.

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    APA: Copy

    NILFOROUSHZADEH, MOHAMMAD ALI, AGHDAMI, NASSER, & TAGHIABADI, EHSAN. (2021). Effects of Adipose-Derived Stem Cells and Platelet-Rich Plasma Exosomes on The Inductivity of Hair Dermal Papilla Cells. CELL JOURNAL (YAKHTEH), 23(5), 576-583. SID. https://sid.ir/paper/682465/en

    Vancouver: Copy

    NILFOROUSHZADEH MOHAMMAD ALI, AGHDAMI NASSER, TAGHIABADI EHSAN. Effects of Adipose-Derived Stem Cells and Platelet-Rich Plasma Exosomes on The Inductivity of Hair Dermal Papilla Cells. CELL JOURNAL (YAKHTEH)[Internet]. 2021;23(5):576-583. Available from: https://sid.ir/paper/682465/en

    IEEE: Copy

    MOHAMMAD ALI NILFOROUSHZADEH, NASSER AGHDAMI, and EHSAN TAGHIABADI, “Effects of Adipose-Derived Stem Cells and Platelet-Rich Plasma Exosomes on The Inductivity of Hair Dermal Papilla Cells,” CELL JOURNAL (YAKHTEH), vol. 23, no. 5, pp. 576–583, 2021, [Online]. Available: https://sid.ir/paper/682465/en

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