Evaluation of Cytotoxic and Apoptotic Effects of DT386– BR2: A  Promising Anticancer Fusion Protein

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SHAFIEE FATEMEH; RABBANI MOHAMMAD; JAHANIAN NAJAFABADI ALI

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Journal Paper

Paper Information

Journal: JOURNAL OF REPORTS IN PHARMACEUTICAL SCIENCES Year:2020 | Volume:9 | Issue:1 Page(s): 68-72

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Title

Evaluation of Cytotoxic and Apoptotic Effects of DT386– BR2: A Promising Anticancer Fusion Protein

Author(s)

SHAFIEE FATEMEH | RABBANI MOHAMMAD | JAHANIAN NAJAFABADI ALI | Issue Writer Certificate

Keywords

Apoptosis

cytotoxicity

DT386–BR2

flow cytometry

K-562

Abstract

Purpose: In the previous studies, we designed an anticancer immunotoxin containing the catalytic and translocation domains of diphtheria toxin fused to BR2, a buforin II-derived antimicrobial peptide as a cancer-specific cell penetrating peptide, in order to target various cancer cells. The aim of this study was to evaluate the in vitro cytotoxicity of DT386– BR2 against K-562 cells as the most famous cell line for leukemia. Materials and Methods: MTT and flow-cytometry assays were used for determining the cytotoxic effects and cell death mechanism of DT386– BR2, respectively, against K-562 cell line. The recombinant DT386 and synthetic BR2 were used as the negative control in cytotoxicity assay. Results: The results of this study showed a significant reduction in survival of K-562 cells caused by DT386– BR2 as compared with BR2 and DT386 fragments. On the contrary, the flow-cytometry results showed Apoptosis induction by DT386– BR2 after 12 h in a dose-and time-dependent manner. Conclusion: DT386– BR2 fusion protein can be used for further preclinical studies for determining its pharmacokinetic/ pharmacodynamic profiles and evaluating its anticancer efficacy in suitable animal models.

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APA: Copy

SHAFIEE, FATEMEH, RABBANI, MOHAMMAD, & JAHANIAN NAJAFABADI, ALI. (2020). Evaluation of Cytotoxic and Apoptotic Effects of DT386– BR2: A Promising Anticancer Fusion Protein. JOURNAL OF REPORTS IN PHARMACEUTICAL SCIENCES, 9(1), 68-72. SID. https://sid.ir/paper/683171/en

Vancouver: Copy

SHAFIEE FATEMEH, RABBANI MOHAMMAD, JAHANIAN NAJAFABADI ALI. Evaluation of Cytotoxic and Apoptotic Effects of DT386– BR2: A Promising Anticancer Fusion Protein. JOURNAL OF REPORTS IN PHARMACEUTICAL SCIENCES[Internet]. 2020;9(1):68-72. Available from: https://sid.ir/paper/683171/en

IEEE: Copy

FATEMEH SHAFIEE, MOHAMMAD RABBANI, and ALI JAHANIAN NAJAFABADI, “Evaluation of Cytotoxic and Apoptotic Effects of DT386– BR2: A Promising Anticancer Fusion Protein,” JOURNAL OF REPORTS IN PHARMACEUTICAL SCIENCES, vol. 9, no. 1, pp. 68–72, 2020, [Online]. Available: https://sid.ir/paper/683171/en

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