مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

GENOTYPING OF HEPATITIS B VIRUS BY MULTIPLEX PCR IN SISTAN AND BALUCHESTAN PROVINCE

Pages

  0-0

Abstract

 Background: HEPATITIS B VIRUS (HBV) has been classified into 8 GENOTYPEs (A - H) according to at least 8% difference in complete nucleotide sequences or more than 4% in S gene. Different GENOTYPEs of HBV have important roles in development of certain disorders as: hepatitis, cirrhosis and hepatocellular carcinoma. Therefore, knowing the distribution of HBV GENOTYPEs, have considerable impacts on treatment strategies, vaccination program, diagnosis and prevention.Objectives: This study aims to determine the prevalence of HBV GENOTYPEs in patients infected with the virus in Sistan and Baluchestan province (south-east Iran).Patients and Methods: In this descriptive study, 163 patients infected with HBV were selected over the first 6 months (beginning from March 2012 onward). All the patients were HBsAg (hepatitisBsurface antigen) and HBeAg (extracellular form of HBcAg) positive along with viral load> 10000 copies/mL. After DNA extraction from the patients’ sera, viral GENOTYPEs were determined by MULTIPLEX POLYMERASE CHAIN REACTION (PCR) using special primers for each GENOTYPE.Results: From 163 samples, 154 samples (94.5%) had D GENOTYPE which was dominant GENOTYPE, and 9 samples (5.5%) had mixed GENOTYPE of C/D.Conclusions: This study showed that in Sistan and Baluchestan, like other regions of Iran as well as Mediterranean countries, D GENOTYPE is dominant GENOTYPE. In additional, C/D mixed GENOTYPE is also seen less frequently in this province.

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    APA: Copy

    YOUSEFI, KAJAL, TAHERI, MOHSEN, KHOSRAVI, SOHEILA, SANEI MOGHADAM, ESMAIL, & MOHAGHEGHIFARD, AMIR HOSSEIN. (2016). GENOTYPING OF HEPATITIS B VIRUS BY MULTIPLEX PCR IN SISTAN AND BALUCHESTAN PROVINCE. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 18(2), 0-0. SID. https://sid.ir/paper/683557/en

    Vancouver: Copy

    YOUSEFI KAJAL, TAHERI MOHSEN, KHOSRAVI SOHEILA, SANEI MOGHADAM ESMAIL, MOHAGHEGHIFARD AMIR HOSSEIN. GENOTYPING OF HEPATITIS B VIRUS BY MULTIPLEX PCR IN SISTAN AND BALUCHESTAN PROVINCE. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2016;18(2):0-0. Available from: https://sid.ir/paper/683557/en

    IEEE: Copy

    KAJAL YOUSEFI, MOHSEN TAHERI, SOHEILA KHOSRAVI, ESMAIL SANEI MOGHADAM, and AMIR HOSSEIN MOHAGHEGHIFARD, “GENOTYPING OF HEPATITIS B VIRUS BY MULTIPLEX PCR IN SISTAN AND BALUCHESTAN PROVINCE,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 18, no. 2, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/683557/en

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